Submerged Cultivation of Ganoderma lucidum for the Production of Bioactive Components and the Anti-allergic Function of the Fermentation Products
Date Issued
2005
Date
2005
Author(s)
Chen, Shiau-Wei
DOI
zh-TW
Abstract
Abstract
The medium consisting of B plants, C and A, was used for cultivating G. lucidum. It was found that cultivation of G. lucidum in 500 ml flask with the medium consisting of 5% C, 2% A ( A ), and 2% glucose yielded the highest amounts of polysaccharide and (1→3)-β-D-glucan. Low inoculation density could promote the production of (1→3)-β-D-glucan and crude triterpenoids during submerged fermentation at x℃, aeration rates も vvm, and agitation speed もrpm in a 200L fermenter. Due to the environment and temperature restriction, the two-stage fermentation could improve crude triterpenoids production. And fed batch cultivation could further increase the yield of polysaccharide and (1→3)-β-D-glucan in the fermentation broth.
For the anti-allergic effect, the mononuclear cells (MNCs) from human was used. Results indicated that the fermented products could reduce the mRNA expression of the TH1 cytokines IL-4, IL-5, IL-13 by the mitogen-activated MNCs. Although the fermented products also reduced the mRNA expression of the TH2 cytokines IFN-γand IL-2, the extend of reduction was less than that mRNA expression of the TH1 cytokines.
Subjects
靈芝
深層液態醱酵
Ganoderma lucidum
submerged fermentation
Type
thesis
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