Characteristics of Water-Soluble Polysaccharides from Agaricus blazei Murill
Date Issued
2005
Date
2005
Author(s)
Yang, Li-Ying
DOI
zh-TW
Abstract
Abstract
To investigate composition and structural characteristics of water-soluble polysaccharides of Agaricus blazei Murill, three enzymatic systems were applied to quantity the contents of (1,4; 1,6)-a-D-glucans, (1,6)-b-D-glucans and (1,3 )-b-D-glucans in the hot-water extracts of fruiting bodies. Molecular size distribution of these three D-glucans was revealed by size-exclusion chromatography and dye staining techniques. Polysaccharides from mycelium of this mushroom obtained from liquid culture were also studied for comparison. The dried fruiting bodies contained 9.1% of soluble dietary fibers (SDF) and had 26.4% nitrogen free extract. The yields of hot-water extracts and of crude polysaccharides were 66.8% and 18.7% of the solid, respectively. The crude polysaccharides contained 94% of glucose and 6% galactose with trace amount of fucose and mannose. The crude polysaccharides consisted of 40% of (1,4; 1,6)-a-D-glucans, 28% of (1,6)-b-D-glucans and 2.2% of (1,3 )-b-D-glucans and other polysaccharides containing galactose, fucose and mannose. The (1,4; 1,6)-a- D-glucans had molecular weight distribution in the range of 177~1,000 kDa and did not react with iodine solution to form blue or purple color. We proposed that the a-D-glucans had a branch-on-branch structure with high branching ratio. The (1,6)- -b-D-glucans were found to be linear molecules which could convert to gentiobiose, gentiotriose and small amount of gento-oligosaccharides using (1, 6)-b-D-glucanase and molecular weight distribution in the range of 6 ~ 800 kDa. The small amount of (1, 3 )-b-D- glucans were essential linear molecules with short glucosyl branches. The degree of branching was found to be 0.15.
The polysaccharides obtained from mycelium culture contained high proportion of galactose and mannose consisting of about 85% of sugar components with appreciable amount of fucose and glucose and arabinose and were significantly different from polysaccharides from fruiting bodies. Adding 2% of blackbean (Glycine max) as medium significantly increased total polysaccharides yield of products. The inference of blackbean polysaccharides in the culture broth caused the difficulty of monitoring on the production of A. blazei polysaccharides. The fucose content in polysaccharide could serve a good index which increased as cultivating time increasing.
Subjects
巴西洋菇
(1,4
1,6)-D-葡萄聚醣
(1,6)-D-葡萄聚醣
(1,3)-D-葡萄聚醣
Agaricus blazei Murill
1,6)-D-glucans
(1,6)-D-glucans
(1,3)-D-glucans
Type
thesis
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