The immunomodulatory function of the polysaccharides produced by fed-batch fermentation of Ganoderma lucidum
Date Issued
2007
Date
2007
Author(s)
Lee, Yen-Jui
DOI
zh-TW
Abstract
The objectives of this research were to determine the optimum fermentation condition by using the content of (1→3)-β-D-glucans and different molecular weight of polysaccharides as the index of the immunomodulating effects. Black soybean was used as the major cultivating media for Ganoderma lucidum with addition of different substrates including Astragalus membranaceus, glucose and polyoxin-B during fed-batch fermentation. Different concentrations of ethanol were used to separate the fermented filtrate into two parts: large and small molecular weight polysaccharides solution, which were further used to test the inhibitory ratio using leukemic U937 cell and the content of TNF-α, IL-1β and IFN-γ in the conditioned medium.
The results indicates that during cultivation of G. lucidum using the feed of A. membrabaceus gives high content of (1→3)-β-D-glucans with better immunomodulating effects. Using different ethanol fraction of polysaccharides, it was observed that 40% ethanol separate the large molecular weight of polysaccharides out of the fermented filtrate. Also these large molecular weight polysaccharides solutions were having higher contents of (1→3)-β-D-glucans, and better immunomodulating effects then the small molecular weight of polysaccharides solutions.
Subjects
靈芝
餽料式發酵
多醣
(1→3)-beta-D-葡萄聚醣
免疫活性
Ganoderma lucidum
fed-batch fermentation
polysaccharide
(1→3)-β-D-glucans
immunomodulating effects
Type
thesis
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