dc.description.abstract | Long-term inflammation has been proven that is related to chronic diseases such as cancer, asthma, and atherosclerosis. Adlay (Coix lachryma-jobi L var. ma-yuen Stapf) was used as food and medicine, and many researches pointed out that adlay possess immuno-mediating, hormone-regulating, anti-oxidative, anti-inflammation, and anti-cancer effects. To promote functional materials utilized comprehensively, this study supposed adlay as a nutritional supplement to evaluate the stability according to Health Food Management Law published by Department of Health. In 6 months experimental period, time points at 0, 3, and 6 month or 0, 1, 2, 3, and 6 month, the samples of ABE (adaly bran ethanolic extract) and sub-fractions (ABE-Hex, ABE-Ea, and ABE-Bu) were taken to quantify the changes of components possessed anti-inflammation activity during storage period. 8 phenolic compounds and 3 phytosterols were chosen as targets, the former comprised included chlorogenic acid, vanillic acid, caffeic acid, p-coumaric acid, ferulic acid, 4-hydroxyacetophenone, syringaldehyde, and 6-methoxy-2-benzoxazolinine, while the latter comprised campesterol, stigmasterol, and β-sitosterol. Meantime, peroxide value (POV) was used to evaluate sample stability during storage periods. The results showed that POV in accelerating test were higher in ABE and ABE-Hex than other fractions at 0 month, and became lower as storage time passed. POV in ABE-Bu was stable and low at all time points. Phenolic compounds are abundant in ABE and sub-fractions, which located at ABE-Ea and ABE-Bu, are the most among all groups. As storage time passed, phenolic compounds contents changed in all fractions, and after 6 month all of the phenolic compounds significantly decreased. Values in phytosterols pointed out that β-sitosterol occupied the most quantity in all fractions, while ABE and ABE-Hex contained most phytosterols. After storage periods phytosterol contents decreased and at the time point 6 month the contents lowered significantly. Above all, the active compounds in ABE and fractions changed as storage time passed by, so the storage environment shall be noticed that to ensure quality of extracts. Still, qualified and quantified compounds in this study are active compounds that possess anti-inflammatory effect and ferulic acid may be mainly compound in ABE-Ea subfractions contributed to anti-inflammation. | en |
dc.description.tableofcontents | 摘要 IBSTRACT II要名詞中英文對照表 III錄 V目錄 IX目錄 XI、 前言 1、 文獻回顧 7、 『薏苡』、『糙薏仁』、『薏仁麩皮』 7一) 基本簡介 7. 『薏苡』背景資料 7. 『薏苡』基本結構組成 7. 『糙薏仁』、『薏仁麩皮』營養組成 8二) 『薏苡』之特殊生理機能性物質與含量 9三) 保健食品 10. 定義 10. 『糙薏仁』、『薏仁麩皮』發展成保健食品之潛力: 141) 免疫調節和輔助調整過敏體質 142) 調節血脂和血糖作用 153) 調整腸胃功能 164) 延緩衰老 (抗氧化能力) 175) 調節血壓 186) 其他 18. 美白抗氧化 18. 調節激素作用 19. 抗致突變性 19. 輔助抑制腫瘤 19、 發炎反應 (Inflammation) 22一) 環氧化酵素 (Cyclooxygenase, COX) 23二) 一氧化氮合成酵素 ( nitric oxide synthase, NOS ) 23三) 結腸直腸癌與發炎 24、 放射治療與發炎反應 25、 與抑制發炎反應相關之生理活性物質 30一) 植物固醇 30二) 酚類化合物 37、 與樣品安定性與品質相關之指標 41一) 油脂劣變反應 41. 水解作用(Lipolysis) 41. 自氧化作用 (Autoxidation) 41二) 油脂初期劣變指標-過氧化價 ( peroxide value, POV ) 43三) 影響油脂過氧化之因子-包裝之考量因素 46四) 預防油脂過氧化之方法-包裝之考量因素 46、 研究目的與實驗設計 47、 研究目的 47、 實驗設計 48、 實驗架構 51、 實驗材料與方法 52、 實驗材料 52一) 薏仁麩皮萃取物及其次區分層 52二) 化學藥品與溶劑 56. 化學藥品 56. 溶劑 56. 其他 56三) 標準物質 56四) 儀器設備 57. 樣品貯存機器 57. 樣品分析設備 57、 實驗方法 58一) 安定性試驗 58. 加速試驗、長期試驗 581) 加速試驗 (Accelerated testing) 582) 長期試驗 (Long term testing) 58. 安定性試驗條件 58. 有效期間 59. 本試驗的條件與設計 59二) 品質指標分析-過氧化價 (Ferric thiocyanate method) 60三) 生理活性成份 64. 酚類物質 64. 分析條件 64. Calibration curve 65. 植物固醇 66. 分析條件 67. Calibration curve 67、 結果與討論 69、 產品過氧化物含量的變化-過氧化價(PEROXIDE VALUE) 69、 植物固醇含量的變化 74、 酚類物質含量的變化 83、 樣品-薏仁麩皮乙醇萃取物乙酸乙酯區分物之次區分物有效期限之推算 105、 結論 108、 參考文獻 109 | en |