Effects of lactic acid bacteria fermented buckwheat extracts on the antiproliferation of colon cancer cell line HT-29 and anticytotoxicity of human intestine cell line Int-407
Date Issued
2012
Date
2012
Author(s)
Liao, Tasi-Ru
Abstract
Buckwheat (Fagopyrum esculentum Moench) is abundant in nutrients, and contains antioxidants, such as rutin, quercetin, polyphenols and flavonoids. Buckwheat has a lot of functional activities of such as antioxidative and antitumor activities. Study has shown that probiotics has many beneficial effects on human health such as strengthening immunity regulation, decreasing the toxigenic/mutagenic reactions in the gut and lower the risk of colon cancer. The aim of this study was to evaluate the anticytotoxic and antigentoxic effects of the extracts of fermented buckwheat and buckwheat sprouts. Results showed that the fermented 5-day germinated buckwheat sprouts were the chioce fermented material for the starters (L. acidophilus BCRC 14079、L. casei 01、L. plantarum BCRC11697、L. rhamnosus GG BCRC 16000、B. infantis BCRC14602、B. longum BCRC14634). The extracts of fermented buckwheat sprouts, its total phenolic contents, total flavonoid content, DPPH-radical scavenging activity and metal chelating activity were significantly higher (p>0.05) than the extracts of fermented buckwheat. The extracts of fermented buckwheat sprouts of L. casei 01 could reduce the cell viability in HT-29. The lowest in HT-29 for 24, 48h and the viability were 65.8% and 48.6%, respectively. Furthermore, the prevention of 4NQO-induced DNA damage on Int-407 cell line was tested by comet assay. The extracts of fermented buckwheat and buckwheat sprouts of L. casei 01 could reduce the genotoxicity of 4NQO-induced DNA damage on Int-407. The possible anticytotoxic mechanisms of extracts were investigated by section-preincubation test. The major mechanisms of the extracts of fermented buckwheat and buckwheat sprouts of L. casei were blocking effects and bioantitoxic effects.
Subjects
buckwheat
lactic acid bacteria
antiproliferation
anticytotoxicity
SDGs
Type
thesis
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