Effects of Probiotcis-Fermented Supernatant from Substrates Containing Oligosaccharides on the Inhibition of Colon Cancer Cell Proliferation and Antitoxicity of Human Colon Cell Line Int-407
Date Issued
2010
Date
2010
Author(s)
Chiang, Shu-Ching
Abstract
Prebiotics are usually non-digestible oligosaccharides to human. They are resistant to gastric acid and digestive enzymes so that they could reach into the large intestines and were fermented by the intestinal microbes. Recently, some research has focused on the functions of the metabolites and short chain fatty acids that they might suppress the proliferation of colon adenocarcinoma cells. The aim of this study was to evaluate the anticytotoxic and antigentoxic effects of fermented supernatant by Lactobacillus casei 01, Bifidobaterium lactis Bb-12 and Lactobacillus plantarum in inulin, fructooligosacchardie (FOS), isomaltooligosaccharide (IMO) and xylooligosaccharide (XOS) contained medium.
The growth curves showed that three strains of probiotics could grow in the oligosaccharides-contained medium and the pH values were decreasing during the fermentation. The results showed that the fermented supernatant could suppress the proliferation of HT-29 (p < 0.05) except L. plantarum-IMO group. In addition, the XOS-fermented supernatant showed the best suppression. The viability of 4NQO group was 54% and almost of cell viability in the test groups increased by treating with the fermented supernatant (p < 0.05), and the FOS-fermented supernatant showed the best ability in the promoting the cell viability after 4NQO induced cell damages. Furthermore, we observed that the DNA tails reduced in the test groups significantly by comet assay (p < 0.05). From the analysis of organic acids, the XOS-fermented supernatant contained higher concentration of total SCFAs that might contribute to the decreasing viabilities on HT-29. FOS-fermented supernatant contained the highest butyrate concentration which might contribute to the anticytotoxicity and antigenotoxicity.
Subjects
oligosaccharides
fermentation
short chain fatty aicds
colon cancer
anticytotoxicity
SDGs
Type
thesis
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