Anti-skin aging effect of fermented product using Aspergillus oryzae and Saccharomyces cerevisiae
Date Issued
2014
Date
2014
Author(s)
Lai, Man-Ning
Abstract
Skin is the most visible organ in the aging process. The characteristics of skin aging include wrinkling, sallowness, laxity and dryness. Aging may result from both the passage of time (intrinsic aging) and from ultraviolet light exposure (extrinsic aging). Past research showed that sake (rice wine) has positive effect on skin, including prevention of UV damage, reduction of matrix metalloproteinase-1 (MMP-1) expression and increase of typeⅠprocollagen synthesis. However, rice wine production requires tedious procedures and longtime processing. In this study, we attempt to simulate the fermentation conditions of rice wine production but with much shorter time, and hope that the fermentation product also possess anti-aging effect on skin. Since both peptide and carbohydrate are all considered to possess anti-aging effect for skin, other than rice, soybean meal was also used as substrates for fermentation. The fermentation experiment was divided into two parts. First, the optimal composition and fermentation time of koji prepared by Aspergillus oryzae were determined. Then, two kind of Saccharomyces cerevisiae were added to complete the fermentation process. The human skin fibroblast (CCD-966SK) was used as in vitro model to evaluate the bioactivity of the fermentation product. Results showed that the dose below 50μg/mL would not exert cytotoxic effect on the human skin fibroblast. The samples derived from ethanol extract of fermented product which fermented by Aspergillus oryzae and Saccharomyces cerevisiae YCL1087 could significantly reduce about 20％ of the accumulation of UV-induced ROS production in skin fibroblast. But the samples with higher concentration could inhibit typeⅠprocollagen synthesis. If treated with low concentration of the product fermented with Aspergillus oryzae for a shorter time, compared with control group, there was no significant effect on typeⅠprocollagen synthesis. Therefore, this fermented extract could scavenge ROS production significantly and reduce UV-induced oxidative stresses in skin cells. But this product is not a comprehensive skin care product, because of they cannot stimulate typeⅠprocollagen synthesis.
Subjects
皮膚老化
清酒
膠原蛋白
基質金屬蛋白酶
發酵
Type
thesis
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