Optimization of exopolysaccharides production of Ganoderma formosanum using PCS bioreactor and its anti-inflammatory activity
Date Issued
2016
Date
2016
Author(s)
Wu, Shu-Pei
Abstract
Ganoderma formosanum also named tzu-chih, is an endemic species of lingzhi in Taiwan. Recently, researchers had proved that an extracellular polysaccharides (EPS) purified from submerged culture of G. formosanum could exert immune-modulating and anti-tumor effects. For the purpose of improving G. formosanum EPS, plastic composite support (PCS) immobilization system was developed, also, anti-inflammatory effect was evaluated after the prodution. Among four PCS (SF+, SY+, SB+ and SR+) in selection stage, SF+ immobilized system showed the highest stimulation of EPS and biofilm formation; further in optimization process, SF+ combined with different carbon sources in immobilization system demonstrated a significant improvement of EPS production ( p < 0.05 ) in bi-stage fermentation. During continuous fermentation, 5 repeated-times as many as that of conventional suspended-cell bioreactors was contributed to a shorter producing time, easy manufacturing process and lower cost in PCS bioreactors. Bioactivity evaluation demonstrated that a lower radical scavenging concentration of immobilized group EPS than that of the L/G group, while the anti-inflammatory assay showed no significant difference on inhibiting nitric oxide ( NO ) production with 125 μg/mL EPS treatment in RAW 264.7 cells. Analysis of monosaccharide composition and β-1,3-glucan content depicted that β-glucan and fucose-enriched polysaccharides may contribute to the bioactivity on radical scavenging and anti-inflammation of EPS fermented in suspension and SF+ immobilized system respectively.
Subjects
Ganoderma formosanum
anti-inflammation
β-glucan
Type
thesis
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