Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Bioresources and Agriculture / 生物資源暨農學院
  3. Food Science and Technology / 食品科技研究所
  4. Bioethanol Production from Taro Agricultural Resource Using Thermotolerance Yeast Kluyveromyces marxianus K21
 
  • Details

Bioethanol Production from Taro Agricultural Resource Using Thermotolerance Yeast Kluyveromyces marxianus K21

Date Issued
2015
Date
2015
Author(s)
Wu, Wei-Hao
URI
http://ntur.lib.ntu.edu.tw//handle/246246/273944
Abstract
The demand for fossil fuels has significantly increased since the beginning of industrial revolution. Petroleum, one of the most important types of fossil fuel, is predominantly being used as car’s fuel. Fossil fuel dependence leads to the increase of greenhouse gas, resulting in more severe greenhouse effect and global warming. In order to lessen the amount of air pollution due to fossil fuel burning and to alleviate the energy crisis, many countries began to develop alternative fuels. Bioethanol is one of the high efficient and low environmental impact bioenergy sources. The raw materials that produce bioethanol can be divided into sugar, starchy and lignocellulose materials. Using starchy materials for bioethanol production have many advantages, such as rich in fermented sugar, high efficiency of ethanol production, simple operation and so on. Most of starchy materials are foods and have lot of controversies. Second generation bioethanol are using lignocellulosic materials. These materials have some properties, such as harsh hydrolysis conditions, fewer fermented sugar, and lot of inhibition substances. If we can find a kind of starchy agricultural resource, it can compensate for the disadvantages of both. Taro is one of the most important crops in Taiwan and annual output about 40,000 tons, which serves as main ingredient of different types of food products such as pastries, snacks, and dessert. In this study, we used taro agricultural resource (TAR), which is provided by taro pastries factory to perform our experiment. The received TAR was mainly composed of taro’s skins that contain many starch residues. We pretreated and enzymatically hydrolyzed TAR. Various alternative nitrogen sources were evaluated as substitute nitrogen source of yeast extract for producing more economic and environmental friendly medium for ethanol production. Subsequently, we are going to discuss about the factors of modification medium, such as TAR loading amount, ions concentration, inoculum and medium filtration. The modification medium is followed by using thermotolerance yeast K21 to separate hydrolysis fermentation (SHF) and simultaneous saccharification fermentation (SSF) under different temperature. Our ultimate goal is scale-up to 5L bioreactor to make sure of conditions can produce ethanol efficiently by using TAR. The flask scale result showed the optimizing medium was using 170 g/L TAR which can hydrolyze about 100 g/L glucose and added 9 g/L CGM and salt without filtration. The optimizing medium was inoculated 5% K21 under 40oC by SSF can carry out the maximum ethanol concentration (48.98g/L) and productivity (2.23g/L/h). The result of maximum ethanol concentration and productivity were 43.78 g/L and 2.19 g/L/h, respectively in 5L bioreactor under the optimizing condition.
Subjects
Taro agriculture resource
alternative nitrogen source
simultaneous saccharification fermentation
bioethanol
Type
thesis
File(s)
Loading...
Thumbnail Image
Name

ntu-104-R01641023-1.pdf

Size

23.54 KB

Format

Adobe PDF

Checksum

(MD5):88cc562e91bdd7a55984e71887e8fbc7

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science