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  4. Ginger ethanolic extract and its pungent compounds alleviate oleic acid-induced lipid accumulation in normal liver cell line of mouse
 
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Ginger ethanolic extract and its pungent compounds alleviate oleic acid-induced lipid accumulation in normal liver cell line of mouse

Date Issued
2016
Date
2016
Author(s)
Chu, Jia-Ping
URI
http://ntur.lib.ntu.edu.tw//handle/246246/273966
Abstract
Nonalcoholic fatty liver disease (NAFLD) is a common metabolic disorder. The pathogenesis is excessive lipid accumulation in liver and it is related to metabolic syndrome. Ginger (Zingiber officinale Roscoe) belongs to rhizome plants and mainly grows in south Asia. Ginger contains pungent compouds and has been widely used in various foods, beverages, seasoning and traditional Chinese medicine. The ginger ethnaolic extract (GEE) was prepared by immersing ginger powder in 95% ethanol. In the present study, we investigate the effect of GEE and three pungent compounds (6-gingerol, 6-shogaol and zingerone) on oleic acid-induced lipid accumulation for 24 hours in FL83B cells, the normal liver cells of mice. GEE extraction rate was approximately 3.78%. HPLC analysis showed that the content of 6-gingerol, 6-shogaol and zingerone was 102.4, 23.8 and 0.37 mg per gram of GEE, respectively. Nile red staining was used to evaluate the effect of GEE and three pungent compounds on oleic acid-induced lipid accumulation without affecting cell viability measured by MTT assay. The results showed GEE, 6-gingerol and 6-shogaol significantly decreased Nile red fluorescence intensity and intracellular triglyceride content at low concentrations while zingerone only at very high concentration had significant difference. The effect of GEE on alleviating lipid accumulation presumably derived from the activity of 6-gingerol and 6-shoagol. To further elucidate the mechanisms, we conducted Western blotting. The results showed that GEE, 6-gingerol and 6-shogaol not only significantly decreased the expression of SREBP-1, FAS, SREBP-2 and HMGCR, but also promoted AMPK activation results in increasing the phosphorylation of ACC. GEE and 6-gingerol significantly increased the expression of PPARα and CPT-1 but 6-shogaol had no significant difference. On the basis of above results suggested that GEE, 6-gingerol and 6-shogaol may alleviate oleic acid-induced lipid accumulation in FL83B cells by modulation of protein expression associated with lipid oxidation, lipid synthesis and cholesterol synthesis.
Subjects
ginger ethanolic extract
pungent compounds
oleic acid-induced lipid accumulation
FL83B cell
Type
thesis
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ntu-105-R02641037-1.pdf

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