|Title:||Allyl Sulfides Inhibit Cell Growth of Skin Cancer Cells through Induction of DNA Damage Mediated G2/M Arrest and Apoptosis||Authors:||Wang, Hsiao?Chi
|Keywords:||Allyl sulfides; Apoptosis; Basal cell carcinoma; Cell cycle; DNA damage; Melanoma; Skin cancer||Issue Date:||2010||Journal Volume:||58||Journal Issue:||11||Start page/Pages:||7096-7103||Source:||Journal of Agricultural and Food Chemistry||Abstract:||
Diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS), extracted from crushed garlic by steam-distillation, have been reported to provide the anticancer activity in several cancer types. However, their mechanisms of effects on skin cancer cells remain unclear. Therefore, we used human melanoma A375 cells and basal cell carcinoma cells as the models to elucidate the effects of these three allyl sulfides. Basal cell carcinoma (BCC) is known to be the most prevalent type of skin cancer, and melanoma is the most lethal form. We found that DATS revealed better growth inhibition of A375 and BCC cells than DADS and DAS did. We further demonstrated that DATS increased intracellular reactive oxygen species (ROS) generation, induced cytosolic Ca2+ mobilization, and decreased mitochondrial membrane potential (Δψm). Western blot results showed the concordance for the expression of molecules involved in G2/M arrest and apoptosis observed by cell cycle and cell viability analysis. Moreover, we detected the activation of p53 pathway in response to the oxidative DNA damage. DATS also displayed selective target of growth inhibition between skin cancer cells and normal keratinocyte HaCaT cells. Taken together, these results suggest that DATS is a potential anticancer compound for skin cancer. ? 2010 American Chemical Society.
|SDG/Keyword:||allyl compound; allyl sulfide; protein p53; reactive oxygen metabolite; sulfide; apoptosis; article; cell cycle; cell cycle G2 phase; cell division; cell proliferation; DNA damage; down regulation; drug effect; genetics; human; metabolism; pathophysiology; skin tumor; tumor cell line; Allyl Compounds; Apoptosis; Cell Cycle; Cell Division; Cell Line, Tumor; Cell Proliferation; DNA Damage; Down-Regulation; G2 Phase; Humans; Reactive Oxygen Species; Skin Neoplasms; Sulfides; Tumor Suppressor Protein p53; Allium sativum
|Appears in Collections:||食品科技研究所|
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