https://scholars.lib.ntu.edu.tw/handle/123456789/98326
Title: | Radiation-induced changes in gene-expression profiles for the SCC VII tumor cells grown in vitro and in vivo | Authors: | Cook, John A. Chuang, Eric Y. Tsai, Mong-Hsun Coffin, Debbie Degraff, William Sowers, Anastasia L. ERIC YAO-YU CHUANG |
Issue Date: | 2006 | Journal Volume: | 8 | Journal Issue: | 7-8 | Start page/Pages: | 1263-1272 | Source: | Antioxidants & Redox Signaling | Abstract: | SCCVII tumor cells that grow in vitro or in vivo as a solid tumor were used to compare and contrast gene-expression profiles with or without exposure to two doses of ionizing radiation. Exponentially growing SC-CVII cell cultures and tumors (1 cm diameter) were treated with 0, 2, or 10 Gy, and RNA was collected 1, 3, 6, 12, and 24 h after treatment. Growth under in vitro conditions increased the expression of genes associated with the unfolded protein response (UPR) including ATF4, Ero-1 like, and cystathionase. Growth in vivo indicated that the HIF-1a genes were not upregulated, whereas genes such as hemoglobin α and crystallin α were significantly upregulated. Ninety genes of 16K were found to be significantly modulated under either growth condition by radiation treatment. Gene expression was not dose dependent. Sixty percent of these genes exhibited similar modulation under both in vitro and in vivo conditions; however, 29% of the genes were modulated by radiation under in vivo conditions only. Gene-expression profiles for the same tumor cells can differ, dependent on growth conditions, underscoring the influence that the tumor microenvironment exerts on gene expression for both growth of solid tumors and their response to radiation treatment. ? Mary Ann Liebert, Inc. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/190334 http://ntur.lib.ntu.edu.tw/bitstream/246246/190334/1/07.pdf |
DOI: | 10.1089/ars.2006.8.1263 | SDG/Keyword: | activating transcription factor 4; alpha crystallin; cystathionine gamma lyase; hemoglobin A; hypoxia inducible factor 1alpha; RNA; animal cell; article; cancer cell; cancer growth; cell cycle; cell growth; female; gene expression; gene expression profiling; in vitro study; in vivo study; ionizing radiation; mouse; nonhuman; priority journal; radiation dose; radiation response; signal transduction; solid tumor; squamous cell carcinoma; unfolded protein response; Animals; Carcinoma, Squamous Cell; Cell Culture Techniques; Cell Cycle; Cell Line, Tumor; Cell Survival; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Mice; Mice, Inbred C3H; Neoplasm Transplantation; Neoplasms, Experimental; Radiation Dosage; Radiation, Ionizing; Time Factors; Tumor Suppressor Protein p53; Xenograft Model Antitumor Assays; Ero |
Appears in Collections: | 生物科技研究所 |
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