Chen, Ying‐FangYing‐FangChenJiang, Chung‐LinChung‐LinJiangTan, Chi‐LingChi‐LingTanLai, Pei‐HsiangPei‐HsiangLaiFU-JUNG LIN2025-06-092025-06-092025-05-15https://www.scopus.com/record/display.uri?eid=2-s2.0-105004696573&origin=resultslisthttps://scholars.lib.ntu.edu.tw/handle/123456789/729951Breast cancer remains a leading cause of cancer-related mortality among women, with adipocyte–breast cancer interactions playing a critical role in cancer progression. Mammary gland fat contains both white and brown-like adipocytes. While white adipocytes have been associated with aggressive tumor behavior, the impact of brown-like adipocytes on cancer progression remains largely unclear. This study investigated the roles of beige (UCP1high) and white (UCP1low) adipocytes derived from human adipose-derived mesenchymal stem cells within the tumor microenvironment. Triple-negative breast cancer (TNBC) MDA-MB-231 cells exhibited increased migration and invasion when exposed to white (hWCM) or beige (hBCM) adipocyte-conditioned medium, with a more pronounced effect observed with hBCM. Mechanistically, beige adipocytes secreted significantly higher levels of bone morphogenetic protein 4 (BMP4) compared to white adipocytes, which enhanced TNBC cell migration. Inhibition of BMP signaling with Noggin effectively reduced the migration and malignancy of MDA-MB-231 cells induced by hBCM, underscoring the pivotal role of BMP4 in breast cancer progression.enbeige adipocyteBMP4breast cancerTNBCtumor microenvironment[SDGs]SDG3journal article10.1096/fj.202500158R