王金和2006-07-262018-07-092006-07-262018-07-092005http://ntur.lib.ntu.edu.tw//handle/246246/28737目前無減測家禽白血病J 亞型抗原的方法被發展出來，因為群特異抗原無法分別內源性 與外源性的病毒，為了製造對禽白血病J 亞型抗原特異單源抗體，我們選取台灣毒株的 膜蛋白質的N 端(gp85N)，選殖至pRSET 及一個哺乳動物細胞的表現載體，得到2 株單 源抗體mAb14 與mAb22。使用免疫墨點證明2 個抗體皆可偵測到家禽白血病J 亞型抗 原，但只有mAb22 可以抓到家禽白血病J 亞型病毒。以西方墨點、免疫墨點、免疫化學 及免疫螢光證明mAb22 可偵測家禽白血病J 亞型病毒。因此將此抗體用來進行血漿中家 禽白血病J 亞型抗原或病毒的偵測，結果發現可以測到陽性樣本，然其敏感性及特異性 尚待評估。此檢測方法似可以解決控制家禽白血病的癥結所在。There is a global need to develop a specific antigen detection method for subgroup J avian leukosis virus (ALV-J) since the group-specific antigen detection is unable to distinguish exogenous from endogenous viruses. In order to obtain monoclonal antibodies specific to ALV-J, the N-terminus of the surface unit (gp85N) of the envelope protein from a Taiwanese ALV-J was cloned in pRSET and then in a mammalian expression vector for monoclonal antibody production in mice. After selection from hybridoma cells, two monoclonal antibodies, mAb14 and mAb22 were obtained. Both monoclonal antibodies caught specifically to ALV-J and the expressed gp85 protein from ALV-J but not that from subgorup A avian leukosis virus by immunodot assay. Furthermore, mAb22 was found to catch ALV-J by immunodot, Western blot, immunoperoxidase and immunofluorescent assays. Thus, mAb22 was used for detecting ALV-J antigen or virus in blood. The result shows that this monoclonal antibody could detect positive sample. In conclusion, this monoclonal antibody might be useful for the diagnosis of ALV-J virus or ALV-J-derived antigen.application/pdf53266 bytesapplication/pdfzh-TW國立臺灣大學獸醫學系暨研究所reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/28737/1/932313B002103.pdf