開發奈米金與侵入式耦合連結偵測技術以定量循環腫瘤DNA中TP53基因突變作為食道癌個人化病情監測之臨床檢測平台=Development of a Nanogold and Invasion Coupled Ligation Detection Technology for Quantification of Tp53 Gene Mutation in Circulating Tumor Dna as a Clinical Test Platform for Personalized Disease Monitoring in Esophageal Cancer

2024-06-1T2025-04-21https://scholars.lib.ntu.edu.tw/handle/123456789/728300目前臨床上仍缺乏準確、可負擔的病患特異性循環腫瘤生物標記，以偵測癌症治療後的微量殘存疾病狀態與定期追蹤監測疾病控制狀態。TP53基因突變是癌症最常見的腫瘤基因變異，且突變位點具有病患特異性的特徵。本計畫針對食道鱗狀細胞癌，以創新的奈米金技術結合侵入式耦合連結偵測方法，開發可定量化病患特異性TP53基因變異之液態生檢臨床檢測平台。計畫目標在開發具備高敏感度、高特異性、高再現性、可定量化、可負擔、具方便性的病情監測精準醫療平台。並預計未來可擴展至肺癌、大腸直腸癌、乳癌、頭頸癌等其他癌症之追蹤監測。TP53基因突變病患特異性生物標記；食道鱗狀上皮癌；化學放射治療；奈米金技術入式耦合連結偵測檢測微量殘存疾病循環腫瘤去氧核醣核酸精準醫療疾病追蹤監測液態生檢TP53 gene mutationPatient-specific biomarkerEsophageal squamous cell carcinomachemo radiationNanogold technologyInvasive coupled ligation detection assayMinimal residual diseaseCirculating tumor DNAPrecision medicineDisease follow-up monitoringliquid biopsy開發奈米金與侵入式耦合連結偵測技術以定量循環腫瘤DNA中TP53基因突變作為食道癌個人化病情監測之臨床檢測平台=Development of a Nanogold and Invasion Coupled Ligation Detection Technology for Quantification of Tp53 Gene Mutation in Circulating Tumor Dna as a Clinical Test Platform for Personalized Disease Monitoring in Esophageal Cancer