呂勝春2006-07-262018-07-092006-07-262018-07-092005-06-08http://ntur.lib.ntu.edu.tw//handle/246246/23786Chromatin is suppressive in nature to cellular enzymes that metabolize DNA, mainly due to the inherent inaccessibility of the DNA template. Despite extensive understanding of the involvement of chromatin-modifying factors in transcription, role of related activities in DNA replication remains largely elusive. Here we show that the transcription elongation factor FACT is functionally linked to DNA synthesis progression. Its involvement in DNA replication is partly mediated by the stable association with the replicative helicase complex, MCM, and further by the coexistence with MCM on replication origin. Furthermore, reliant on its nucleosome-reorganizing activity, FACT possesses the ability to facilitate chromatin unwinding by the MCM complex, which is otherwise inert on the nucleosomal template. As a consequence, FACT positively regulates DNA replication and S phase progression in vivo, and such replicative role is evolutionarily conserved. Together, our findings identify FACT as an integral and conserved component of the endogenous replication machinery, and further outline a model in which the concerted action of helicase and chromatin-modifying activities promotes chromatin replication.application/pdf462476 bytesapplication/pdfzh-TW國立臺灣大學醫學院分子醫學研究所journal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/23786/1/932320B002105.pdf