Chien, C HC HChienChang, K TK TChangSONG-NAN CHOW2023-02-172023-02-171990-010255-6596https://scholars.lib.ntu.edu.tw/handle/123456789/628331The amplification and expression of the cellular oncogene c-Ki-ras have been detected in twelve tissue specimens of human ovarian adenocarcinoma, one ascites of ovarian cancer and ovarian cancer cell line NIH: OVCAR-3. Four out of the twelve cancer tissue specimens, one specimen of cancer ascites cells and the NIH: OVCAR-3 cancer cell line showed elevated amplification of c-Ki-ras, as compared to the human fibroblast cell line FS4 and normal ovarian tissues. The amplification of the c-Ki-ras gene showed some correlation with clinical stages of ovarian cancer. The levels of c-Ki-ras specific RNA transcripts and ras gene product p21 were also elevated in these four cancer tissue specimens. Surprisingly, a discrepancy between amplification and expression of c-Ki-ras was noted in five of the other eight cancer tissue specimens, in which enhanced expression of c-Ki-ras was present without the accompanying elevated amplification of c-Ki-ras. The results do provide some clues to understanding the possible mechanisms of tumorigenesis in human ovarian cancer.en[SDGs]SDG3journal article16963862-s2.0-0024985424https://api.elsevier.com/content/abstract/scopus_id/0024985424