2011-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/647700摘要：活髓治療發展至今在臨床上仍然沒有理想之材料。隨著組織再生的觀念應用於牙髓病治療，具生物活性之材料如磷酸鈣陶瓷，因具有良好生物相容性及誘導鈣化組織再生能力，於活髓治療上具有相當的潛力，然而其在臨床應用上仍有操作性質差、材料強度不足及硬化時間過長等缺點。根據本研究團隊初步研究結果顯示，特殊製程的磷酸鈣與半水硫酸鈣複合材料可有效改善磷酸鈣類材料於臨床應用之缺點，因此本計畫研究目的在研發新型活髓材料：磷酸鈣複合生醫陶瓷，並進一步藉由化學表面改質法於材料表面接枝生長因子，探討誘導牙本質牙髓組織再生可能機轉與調控模式。本研究計畫分三年：第一年為磷酸鈣陶瓷/半水硫酸鈣複合材料製程之研發以及磷酸鈣陶瓷的表面改質研究，進行材料性質及生物相容性分析; 第二年評估材料以牙髓幹細胞DPSCs為核心的體外研究模型，應用蛋白質體學分析原理方法，評估磷酸鈣陶瓷/半水硫酸鈣以及磷酸鈣負載骨轉型蛋白（BMP）及可誘導骨再生之中藥有效成分Naringin誘導牙本質牙髓組織再生的可能機轉; 第三年以動物實驗進一步評估所研發材料的安全性及有效性。未來更可將本計畫所研發的材料系統及研究架構應用於其他組織修復機制的探討與生醫材料的改良。<br> Abstract: There has not been an ideal material developed for vital pulp therapy in the past decade. Bioactive calcium phosphate ceramics have been used to repair bone defects, owing to their outstanding biocompatibility and bioactivity. However, poor handling property, low material strength and long setting time are their main problems for clinical applications. We attempt to solve these problems with calcium phosphate ceramics mixed with calcium sulphate hemihydrates. With excellent biocompatibility and highly adjustability of calcium phosphate ceramics, we modify the surface with coupling agent of diisocyanate followed by grafting of BMP growth factor and Naringin.This project has been arranged for three years, and the specific aims are as follows: In the first year, we aim to test the physical and chemical properties of different ratios of calcium sulphate hemihydrates in CPCs. Cytotoxicity studies with MTT, LDH and Agar overlay assay will be conducted. In the second year, we plan to investigate the interaction between the composites and cells as well as the evaluation of dentinogenesis using DPSCs in vitro model and regulatory mechanisms using proteomics technology. In the last year, we will evaluate the interrelationship of composites and pulp cells through animal experiments. This project is aimed to understand the mechanisms underlying pulp-dentin, bone regeneration and testing efficacy in vivo of our materials for further improvements.磷酸鈣陶瓷活髓治療牙本質牙髓再生骨轉型蛋白生物相容性Calcium Phosphate CementsPulp-Dentin RegenerationVital Pulp TherapyBMPBiocompatibility