2011-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682524摘要：十字花科根瘤病（clubroot disease of Brassicaceae ），由Plasmodiophora brassicae Woronin所引起，早在13世紀即有本病害之記載。本病害於臺灣發生於夏季高冷蔬菜產區與冬季平地之蔬菜產地，會造成十字花科蔬菜之根瘤，導致寄主生長勢衰弱，甚至死亡，影響蔬菜收穫甚鉅。目前本病害之防治，多以提高土壤之pH值，或施用農藥為主；但土壤pH值過高，將造成土壤鹽鹼化，而影響蔬菜生長，農藥之使用，亦不符合當今無毒農業之潮流，且提高農民作業成本。本計劃的目的，在發展十字花科根瘤病之防治策略，期望利用產生myrosinase的土壤微生物，觀察其分解glucosinolate之效力，並探討其對根瘤病發生的影響，以期將之利用於十字花科根瘤病生物防治；另外，藉由了解本省P. brassicae的生理小種及其分布，與探討根瘤病菌休眠孢子發芽之影響因子，應用於種植品系之選擇，及田間衛生管理之參考。期望本研究之成果，能應用於十字花科根瘤病的田間防治或預防，對於杜絕及防治本病害之發生，具有台灣本土及國際上相當重要之意義。<br> Abstract: Clubroot disease of Brassicaceae, documented back to 13th century, is caused by the soil-borne obligate pathogen Plasmodiophora brassicae Woronin. In Taiwan, this disease occurs in the vegetable growing areas at high elevation during summer and ground areas of northern parts during winter. The occurrence of this disease causes clubroot symptom, growth decline, and even the death of Brassicaceae plants and results in significant yield loss. To control this disease, increasing soil pH and applying agro-chemicals are two current measures. However, these treatments have dramatic impacts on soil and environment. The goals of this research proposal are to develop alternative control methods for this disease. The major aims are to utilize myrosinase-producing soil microbes, determine their effectiveness on degrading glucosinolates and evaluate the potential in controlling clubroot disease, to determine the physiological races of the pathogen and their distributions in Taiwan, and to dissect the factors affecting the germination of resting spors. We hope the results from our studies can be used to control and prevent the occurrence of clubroot disease.十字花科根瘤病Plasmodiophora brassicae生理小種myrosinase硫配醣體異硫氰化物cluberoot disease of BrassicaceaePlasmodiophora brassicaephysiological racemyrosinaseglucosinolateisothiocyanate