2011-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/703271摘要：番茄黃化捲葉病毒(Tomato yellow leaf curl virus, TYLCV) 所誘發之番茄黃化捲葉病毒病(Tomato yellow leaf curl virus disease) 為近年來影響世界番茄生產的嚴重病害，常造成生產損失。臺灣番茄生產近年受番茄黃化捲葉泰國病毒種(Tomato yellow leaf curl Thailand virus, TYLCTHV) 威脅，而尚無抗病品種。本研究擬以亞洲蔬菜研究發展中心選育之番茄抗TYLCTHV 品系CLN2777G-1-5 及感病品種Tomatoll 為試驗材料，經獲毒之銀葉粉蝨(Bemisia tabaci, Whitefly) B-type 或機械方式接種TYLCTHV 後，調查兩品種、系植株於接種後1~28天之株高、葉數、莖徑與病徵，測定葉片之SPAD、Fv/Fm、抗氧化酵素ascorbate peroxidase、catalase、glutathione reductase、superoxide dismutase 活性，並利用全自動二維蛋白快速層析分離系統(ProteomeLabTM PF-2D) 分析葉片蛋白質體，瞭解番茄抗TYLCTHV之相關生理。<br> Abstract: Tomato yellow leaf curl virus disease (TYLCVD) is a serious disease of tomato and results in economic loss. Tomato plants in Taiwan are threatened by Tomato yellow leaf curl Thailand virus (TYLCTHV). There is no resistant cultivar was developed yet. Tomato TYLCTHV-resistant line CLN2777G-1-5 and susceptible cultivar Tomatoll will be used as experimental materials. Whitefly (Bemisia tabaci) B-type and mechanical method will be utilized to transfer TYLCTHV to the plants. In the 1st~28th days after inoculation, plant height, leaf number, stem diameter, and symptom will be investigated. SPAD, and Fv/Fm, and activities of ascorbate peroxidase, catalase, glutathione reductase, and superoxide dismutase will be measured. Leaf proteomics will be analyzed by ProteomeLabTM PF-2D. The related plant physiology with TYLCTHV resistance will be studied.葉綠素螢光抗氧化酵素蛋白質體Fv/Fmantioxidative enzymesproteomics