YA-YU CHIANGStewart, JoannaJoannaStewartGizewski, CarstenCarstenGizewskiEhrhard, PeterPeterEhrhardJanasek, DirkDirkJanasekWest, JonathanJonathanWest2023-09-222023-09-222012-01-019780979806452https://scholars.lib.ntu.edu.tw/handle/123456789/635630The binding of ligands to receptors initiates signal transduction. Our understanding of the early signal transduction events is limited by the poor temporal resolution of current analytical methods. To address this challenge we have developed a microfluidic strategy for capturing these processes with millisecond temporal resolution. A single cell continuous flow processing method based on deterministic lateral displacement principles was developed and used for cell triggering with microsecond precision. Using precisely defined ligand incubation periods the sub-second receptor tyrosine kinase autophosphorylation dynamics were investigated.Deterministic lateral displacement | IGF-1 receptor | Microfluidics | Signal transduction | Single cellsconference paper2-s2.0-84901768247https://api.elsevier.com/content/abstract/scopus_id/84901768247