TZU-PIN LUChuang N.-C.Cheng C.-Y.Hsu C.-A.YI-CHIH WANGLin Y.-H.JEN-KUANG LEECHO-KAI WUHWANG, JUEY-JENJUEY-JENHWANGLIAN-YU LINYeh S.-F.S.KUO-LIONG CHIENJYH-MING JIMMY JUANG2020-11-172020-11-1720170143-5221https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016410433&doi=10.1042%2fCS20160821&partnerID=40&md5=527031e05f3914380120247882994c22https://scholars.lib.ntu.edu.tw/handle/123456789/520954Coronary artery ectasia (CAE) is a disease characterized by abnormally dilated coronary arteries. The mechanism of CAE remains unclear, and its treatment is limited. Previous studies have shown that risk factors for CAE were related to changes in DNA methylation. However, no systematic investigation of methylation profiles has been performed. Therefore, we compared methylation profiles between 12 CAE patients and 12 propensity-matched individuals with normal coronary arteries using microarrays. Wilcoxon's rank sum tests revealed 89 genes with significantly different methylation levels (P <0.05 and ?> π0.1π). Functional characterization using the DAVID database and gene set enrichment analysis indicated that these genes were involved in immune and inflammatory responses. Of these genes 6 were validated in 29 CAE patients and 87 matched individuals with CAE, using pyro-sequencing. TLR6 and NOTCH4 showed significant differences in methylation between the two groups, and lower protein levels of toll-like receptor 6 (TLR6) were detected in CAE patients. In conclusion, this genome-wide analysis of methylation profiles in CAE patients showed that significant changes in both methylation and expression of TLR6 deserve further study to elucidate their roles in CAE. ? 2017. Biochemical Society. All rights reserved.English[SDGs]SDG3Notch4 receptor; toll like receptor 6; Notch receptor; NOTCH4 protein, human; oncoprotein; TLR6 protein, human; toll like receptor 6; adult; Article; clinical article; controlled study; coronary artery ectasia; data base; DNA methylation; enzyme linked immunosorbent assay; female; gene expression; gene function; genome analysis; human; immune response; inflammation; male; microarray analysis; middle aged; priority journal; protein expression; protein protein interaction; pyrosequencing; rank sum test; aged; blood; case control study; coronary artery disease; coronary blood vessel; genetic predisposition; genetics; genome-wide association study; immunology; lesions and defects; pathology; procedures; Adult; Aged; Case-Control Studies; Coronary Artery Disease; Coronary Vessels; Dilatation, Pathologic; DNA Methylation; Female; Genetic Predisposition to Disease; Genome-Wide Association Study; Humans; Inflammation; Male; Middle Aged; Proto-Oncogene Proteins; Receptors, Notch; Toll-Like Receptor 6journal article10.1042/CS20160821281438912-s2.0-85016410433