指導教授：趙玲臺灣大學：化學工程學研究所丘達Kurniawan, Akhtar FikriAkhtar FikriKurniawan2014-11-252018-06-282014-11-252018-06-282014http://ntur.lib.ntu.edu.tw//handle/246246/261166NoneMembrane fluidity, surface negative charge, and formation of membrane phase have been thought to influence calcium oxalate monohydrate (COM) crystal attachment to the cell membrane. Here, we report that COM attachment induces formation of new phases in supported lipid bilayers. The types and properties of these phases depend on the membrane fluidity and the charge of the used fluorescent lipid dye. We observed that a dark phase in the membrane can be induced by COM attachment on the DOPC membrane. This dark phase is a visual proof of the rearrangement of DOPC membrane components to support the crystal attachment. The DOPC molecules might prefer to have better alignment with each other and exclude the impurity, fluorescent TR-DHPE or Bodipy-HPC, from the well aligned phase. By contrast, the dark phase was not found in the DPPC membrane, where rearrangement and alignment of DPPC is probably more difficult since the fluidity is low at room temperature. We used flows in microfluidic channels to show that crystals attaching on the dark phase attach more strongly to the membrane. The COM crystal attachment also leads to the formation of a bright phase which is probably due to the electrostatic interaction between the crystal face and the anionic components in the membrane. Our findings suggest the importance of membrane rearrangement and phase formation in the attachment of COM to the cell membrane.Abstract ii 1. Introduction 1 2. Materials and Methods 8 2.1. Materials 8 2.2. Apparatus 9 2.3. Lipid Preparation 9 2.4. Preparation Of Supported Lipid Bilayer in Polydimethylsiloxane (PDMS) Well 10 2.5. Membrane Incubation in Crystal Solution 11 2.6. Microscopy 12 2.7. Phase Change Time Distribution and Analysis 13 2.8. Microfluidic Channel Test 13 3. Results 16 3.1. Crystal Observations by Optical Microscopy 16 3.2. Membrane Phases Induced by Crystal Attachment 20 3.3. Using Flow in Microfluidic Channels to Test Crystal Attachment Strength 36 4. Discussions 39 5. Conclusions 47 6. References 494631973 bytesapplication/pdf論文公開時間：2014/08/12論文使用權限：同意有償授權(權利金給回饋學校)草酸鈣結晶支撐式脂雙層膜thesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/261166/1/ntu-103-R01524098-1.pdf