A type II arabinogalactan from anoectochilus formosanus for G-CSF production in macrophages and leukopenia improvement in CT26-bearing mice treated with 5-fluorouracil

Yang L.-C.Lu T.-J.Lin W.-C.2019-07-162019-07-1620131741427Xhttps://scholars.lib.ntu.edu.tw/handle/123456789/414033Anoectochilus formosanus is an herb well known in Asian countries. The polysaccharide isolated from A. formosanus consists of type II arabinogalactan (AGAF), with branched 3,6-Gal as the major moiety. In this study, AGAF was examined for the granulocyte colony-stimulating factor (G-CSF) production and related protein expression in RAW 264.7 murine macrophages. The signaling pathway of G-CSF production involves AGAF and mitogen-activated protein kinases (MAPKs) inhibitors and pattern-recognition receptor antibodies. AGAF was evaluated to ease the leukopenia in CT26-colon-cancer-bearing mice treated with 5-fluorouracil (5-FU). The results of this study showed that AGAF was a stimulant for Toll-like receptor 2 and Dectin-1 and that it induced G-CSF production, through p38 and ERK MAPK, and NF-B pathways. In vivo examination showed that the oral administration of AGAF mitigated the side effects of leukopenia caused by 5-FU in colon-cancer-bearing mice. In conclusion, the botanic type II AGAF in this study was a potent G-CSF inducer in vivo and in vitro. ? 2013 Li-Chan Yang et al.[SDGs]SDG3arabinogalactan; dectin 1; fluorouracil; granulocyte colony stimulating factor; mitogen activated protein kinase; pattern recognition receptor; toll like receptor 2; animal cell; animal experiment; animal model; Anoectochilus formosanus; article; cell viability; colon cancer; comparative study; controlled study; cytokine production; enzyme phosphorylation; herb; leukocyte; leukocyte count; leukopenia; macrophage; male; mouse; nonhuman; priority journal; protein expression; spleen weight; Western blottingA type II arabinogalactan from anoectochilus formosanus for G-CSF production in macrophages and leukopenia improvement in CT26-bearing mice treated with 5-fluorouraciljournal article10.1155/2013/458075https://www.scopus.com/inward/record.uri?eid=2-s2.0-84885439360&doi=10.1155%2f2013%2f458075&partnerID=40&md5=186919fe4544361af980588bdf4545242-s2.0-84885439360https://www.scopus.com/inward/record.uri?eid=2-s2.0-84885439360&doi=10.1155%2f2013%2f458075&partnerID=40&md5=186919fe4544361af980588bdf454524