Wang, H.-K.H.-K.WangTsai, C.-H.C.-H.TsaiChen, K.-H.K.-H.ChenTang, C.-T.C.-T.TangLeou, J.-S.J.-S.LeouLi, P.-C.P.-C.LiTang, Y.-L.Y.-L.TangHsieh, H.-J.H.-J.HsiehWu, H.-C.H.-C.WuCheng, C.-M.C.-M.ChengHSYUE-JEN HSIEH2020-01-062020-01-062014https://scholars.lib.ntu.edu.tw/handle/123456789/445251Here, two types of cellulose-based in vitro diagnostic devices are demonstrated for the diagnosis of dengue virus infection in both buffer system and human serum: 1) paper-based ELISA for providing the semiquantitative information of the disease activity of serotype-2 dengue fever to healthcare persons (i.e., monitoring the disease activity with a specific serotype in single patients); 2) lateral flow immunoassays to screen for infection with serotype-2 dengue fever (i.e., rapid YES or NO diagnosis prepared for large populations, in terms of global public health). Paper-based ELISA (specific to serotype-2 dengue fever), which builds off of our previous studies and a revised previous ELISA procedure, owns multiple advantages: 1) high sensitivity (about 40 times higher than the current ELISA-based approaches, due to our therapeutic-based monoclonal antibody) and specificity (specific to dengue virus serotype-2 nonstructural protein-1 antigens); 2) tiny amount of sample and reagent used for single tests; 3) short operating duration (i.e., rapid diagnostic device); and, 4) inexpensiveness (appropriate for use in all developing and underdeveloped nations of the world). Due to the higher sensitivity and shorter operating duration of paper-based ELISA (compared with conventional ELISA, and lateral flow immunoassays also performed in this study), this study has not only been able to perform the diagnosis of dengue virus serotype-2 nonstructural protein-1 antigens in both buffer system and human serum but also to evaluate dengue virus serotype-2 envelope proteins in the buffer system, thus successfully achieving the first such use of these proteins as the target antigen for the development of diagnostic tools. These results provide a more comprehensive understanding for the genesis of dengue fever diagnostic tools (through antibody-antigen recognition). ? 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Cellulose-based ELISA; Dengue fever; Lateral flow immunoassays; Rapid diagnosis[SDGs]SDG3Antigen-antibody reactions; Antigens; Cellulose; Diagnostic products; Monoclonal antibodies; Viruses; Dengue fevers; Diagnostic device; Diagnostic tools; Envelope proteins; Global public health; In-vitro diagnostic; Lateral flow immunoassay; Nonstructural proteins; Diagnosis; buffer; dengue virus serotype 2 nonstructural protein 1 antigen; unclassified drug; virus antigen; virus protein; antigen antibody reaction; article; dengue; diagnostic test accuracy study; disease activity; ELISA kit; health care cost; hematology test kit; human; immunoassay; in vitro study; intermethod comparison; nonhuman; paper based ELISA kit; priority journal; protein structure; sensitivity and specificity; serotype; serotype 2 dengue fever; virus envelope; cellulose-based ELISA; dengue fever; lateral flow immunoassays; rapid diagnosis; Cellulose; Dengue; Enzyme-Linked Immunosorbent Assay; Humans; Immunoassayjournal article10.1002/adhm.2013001502-s2.0-84893469124https://www.scopus.com/inward/record.uri?eid=2-s2.0-84893469124&doi=10.1002%2fadhm.201300150&partnerID=40&md5=ed5dbf8983acd4812d9360cab7fde35b