National Taiwan University Department of Bio-Industrial Mechatronics EngineeringLu, Fu-MingFu-MingLuChang, Fu-ShiangFu-ShiangChangChen, Po-ChungPo-ChungChenChen, L.C. RichieL.C. RichieChenTZONG-JIH CHENG2006-11-152018-07-102006-11-152018-07-102006-02-07http://ntur.lib.ntu.edu.tw//handle/246246/2006111501264990A stopped-flow manifold was developed to assay and characterize immobilized tannase (EC 3.1.1.20). The immobilized enzyme reactor was inserted within the tube-type electrode pair (cell constant=103.2 cm−1) for a real-time conductometric measurement. Tris buffer (2 mM, pH=7.0) was used as the carrier for sensitivity improvement. The activities and kinetic parameters (Km values) for propyl gallate, methyl gallate and tannic acid were investigated.application/pdf162529 bytesapplication/pdfzh-TWConductometricEnzyme kineticsTannic acidStopped-flowjournal article10.1016/j.bioelechem.2005.12.0042-s2.0-33747827520http://ntur.lib.ntu.edu.tw/bitstream/246246/2006111501264990/1/193.pdf