Lo K.-J.Lin S.-S.Lu C.-W.Kuo C.-H.CHI-TE LIUSHIH-SHUN LIN2020-02-102020-02-10201820452322https://scholars.lib.ntu.edu.tw/handle/123456789/456638Rhodopseudomonas palustris strains PS3 and YSC3 are purple non-sulfur phototrophic bacteria isolated from Taiwanese paddy soils. PS3 has beneficial effects on plant growth and enhances the uptake efficiency of applied fertilizer nutrients. In contrast, YSC3 has no significant effect on plant growth. The genomic structures of PS3 and YSC3 are similar; each contains one circular chromosome that is 5,269,926 or 5,371,816 bp in size, with 4,799 or 4,907 protein-coding genes, respectively. In this study, a large class of genes involved in chemotaxis and motility was identified in both strains, and genes associated with plant growth promotion, such as nitrogen fixation-, IAA synthesis- and ACC deamination-associated genes, were also identified. We noticed that the growth rate, the amount of biofilm formation, and the relative expression levels of several chemotaxis-associated genes were significantly higher for PS3 than for YSC3 upon treatment with root exudates. These results indicate that PS3 responds better to the presence of plant hosts, which may contribute to the successful interactions of PS3 with plant hosts. Moreover, these findings indicate that the existence of gene clusters associated with plant growth promotion is required but not sufficient for a bacterium to exhibit phenotypes associated with plant growth promotion. © 2018, The Author(s).[SDGs]SDG2article; biofilm; case report; chemotaxis; chromosome; clinical article; deamination; gene cluster; gene expression; growth rate; human; nitrogen fixation; nonhuman; phenotype; plant growth; Rhodopseudomonas palustris; root exudate; synthesis; whole genome sequencing; Brassicaceae; chromosomal mapping; comparative study; drug effect; gene expression regulation; genetics; microbiology; multigene family; phylogeny; physiology; plant development; plant genome; plant root; Rhodopseudomonas; carbon; nitrogen; Biofilms; Brassicaceae; Carbon; Chromosome Mapping; Gene Expression Regulation, Bacterial; Genome, Plant; Multigene Family; Nitrogen; Nitrogen Fixation; Phylogeny; Plant Development; Plant Roots; Rhodopseudomonas; Whole Genome Sequencingjournal article10.1038/s41598-018-31128-82-s2.0-85052214421https://www2.scopus.com/inward/record.uri?eid=2-s2.0-85052214421&doi=10.1038%2fs41598-018-31128-8&partnerID=40&md5=4c5837efd6fc3424154b441a087d5a6f