Chen H.-L.Lin K.-W.Huang A.-M.Tu H.-Y.Wei B.-L.Hour T.-C.Yen M.-H.YEONG-SHIAU PULin C.-N.2021-02-022021-02-0220100021-8561https://www.scopus.com/inward/record.uri?eid=2-s2.0-77949778631&doi=10.1021%2fjf903833a&partnerID=40&md5=7eb1947479e67f6b204c2533b80a5df8https://scholars.lib.ntu.edu.tw/handle/123456789/544441Bioguided fractionation of the CHCl3 extracts obtained from Celastrus kusanoi stems led to isolation of two new terpenoids, 3β-hydroxy-11,14-oxo-ableta-8,12-diene (1) and 3β-trans-(3,4- dlhydroxyclnnamoyloxy)-11α-methoxy-12-ursene (2), and four known compounds characterized by spectroscopic methods. Compounds 1 and 2 and known triterpenoid erythrodiol (3) exhibited cytotoxic activity against bladder cancer cells (NTUB1) with IC50 values of 58.2 ± 2.3, 160.1 ± 60.9, and 18.3 ± 0.5 μM, respectively. Exposure of NTUB1 to 3 (5 and 10 μM) for 24 h significantly increased the level of production of reactive oxygen species (ROS). Flow cytometric analysis showed that treatment of NTUB1 with 3 led to the cell cycle arrest at G0/G1 accompanied by an increase in the extent of apoptotic cell death after 24 h. These data suggest that the presentation of G1 phase arrest and apoptosis in 3-treated NTUB1 for 24 h was mediated through an increased amount of ROS in cells exposed to 3. ?2010 American Chemical Society.Celastrus kusanoi; Cytotoxicity; Terpenolds[SDGs]SDG3plant extract; reactive oxygen metabolite; terpene; apoptosis; article; bladder tumor; Celastrus; cell cycle; chemistry; drug effect; human; metabolism; pathophysiology; plant stem; tumor cell line; Apoptosis; Celastrus; Cell Cycle; Cell Line, Tumor; Humans; Plant Extracts; Plant Stems; Reactive Oxygen Species; Terpenes; Urinary Bladder Neoplasms; Celastrusjournal article10.1021/jf903833a201783912-s2.0-77949778631