2008-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/677576摘要：本計劃主旨為研發創新價值之DNA 微全分析系統，突破傳統高成本、多費時之檢測流程。主要分析流程順序為：生醫流體(DNA)進樣、生醫流體與奈米球體之接合、奈米流體與 Target DNA 之混合(包含主動混合、被動混合以及數位液滴操控)以及分離篩選之檢測系統。研發主軸包含幾部份，(1) 發明結合奈米流體之DNA 檢測技術，可有效 避開目前PCR 加熱循環(thermal cycling)的增生專利，進而開發檢測DNA 的微流體裝置，以供應本系統之生化反應、分離篩選與檢測。(2) 微生化流體混合裝置之研發，設計創新之微流道結構或主動混合元件，讓生醫檢體(DNA)與奈米金球產生快速混合作用，以提升後續檢測之準確性及效率。過程中將開發流體混合相關之指標性技術，包含 模擬分析、最佳化設計、混合理論以及系統化之測量技術。(3) 數位生化流體之操控平台的開發，取代傳統之連續進樣，以達成準確定位、低樣品消耗以及快速反應。 此計劃包含了範疇遍及材料合成、微機電、微電子、生化檢測分析以及微熱流領域，除了具有前瞻卓越之學術創新意義外，預期可獲得國際頂尖期刊數篇、國內外專利以及商業化之潛力價值，並成為國內發展微全分析系統之先驅，提供相關領域發展所需之技術。<br> Abstract: The aim of this proposal is to develop a novel a micro-TAS system for DNA detection. It has the advantages for less processing time and lower price than traditional DNA detection approaches. This system includes four major procedures, bio-sample transportation, conjugate of biomolecules with nanoparticles, separation of target biomolecules from samples, and detection of biomolecules. To achieve the aims of this system, this proposal will devote the research efforts on following directions: (1) to propose one novel idea by conjugating nanoparticles with biomolecules for reactions between biomolecules, separation, and DNA detection in order to avoid the patents claimed by major pharmaceutical companies; (2) to develop an advanced micromixer in which some novel designs of microchannel or novel active approaches will be applied. In this advanced micromixer, the mixing efficiency between the sample flow and nanofluid with gold nanoparticles can be significantly enhanced. It can improve the detection sensitivity in later detection procedure. The major techniques developed for improving the performance of this advanced micromixer include numerical simulation, optimal design, mixing analysis and measuring techniques that are expertise of our teams. (3) This project will develop a biochip that serves a platform for digital manipulation of liquid samples. Then, the sampling handling in the micro-TAS system is discrete not continuous. The advantages for using digital manipulation of samples are to reduce the sample consumption and accurate positioning of sample droplets. This project can integrate the technologies in several different areas, such as material, MEMS, microelectronics, biotechnologies, and thermal fluid flow in Microsystems. In additional to the novel ideas proposed in this proposal, we expect to publish several papers in prestigious international journals and to apply some patents after the aims of this proposal are achieved. Our team can be the pioneer for developing a commercial available micro-TAS and can provide some crucial techniques to researches in bio-systems community in Taiwan.微流體混合器雜交生醫檢測mixing devicehybridizationmicromixerconfocal microscopemicro PIV