Li J.G.-R.Chang W.H.-S.Lin J.C.-A.JUI-SHENG SUN2020-02-072020-02-0720020301-5629https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036082502&doi=10.1016%2fS0301-5629%2802%2900485-4&partnerID=40&md5=54745501d0754cb8f1e3081ff7ee1c43https://scholars.lib.ntu.edu.tw/handle/123456789/455403This study compared the effects of different intensity ultrasound (US) on osteoblasts in the far-field model with effects of the near-field model from the literature, to understand the relations between prostaglandin E2 (PGE2) and osteoblast growth. We used an in vitro model to investigate the effects of 1-MHz, pulsed 1:4, and five different spatial-average temporal-peak intensity (150, 300, 600, 1200 and 2400 mW/cm2) US stimulations in far-field exposure (240 mm) on osteoblasts for 15 min. Optimum intensity in this study was 600 mW/cm2, and cell density and PGE2 secretion could be significantly stimulated at this intensity. This research may indicate that the growth of osteoblasts by US stimulation was, at least partly, due to increases in the synthesis and secretion of PGE2. This well-controlled model can lead to further research on the biologic mechanisms for US. ? 2002 World Federation for Ultrasound in Medicine & Biology.[SDGs]SDG3Cells; Hormones; Spatial variables measurement; Osteoblasts; Ultrasonic applications; prostaglandin E2; animal cell; article; cell count; cell density; cell growth; cell stimulation; controlled study; experimental model; nonhuman; osteoblast; priority journal; prostaglandin release; rat; sound intensity; ultrasound; Animals; Cell Count; Humans; Models, Animal; Osteoblasts; Prostaglandins E; Rats; Rats, Wistar; Ultrasonicsjournal article10.1016/S0301-5629(02)00485-4120797052-s2.0-0036082502