WANG-HUEI SHENGYU-TSUNG HUANGSHAN-CHWEN CHANGPO-REN HSUEH2020-03-272020-03-2720090095-1137https://scholars.lib.ntu.edu.tw/handle/123456789/480193We describe a previously healthy patient with chronic otitis media complicated with cerebellar abscess caused by Tsukamurella tyrosinosolvens. The organism was identified based on conventional biochemical identification methods, PCR-restriction fragment length polymorphism analysis of the hsp65 gene, and 16S rRNA gene sequencing. The patient was treated successfully with debridements and prolonged antibiotic therapy. Copyright ? 2009, American Society for Microbiology. All Rights Reserved.[SDGs]SDG3amikacin; amoxicillin; ciprofloxacin; clarithromycin; cotrimoxazole; flomoxef; imipenem; Actinomycetales; adult; article; bacterium identification; brain abscess; case report; clinical feature; female; human; mastoidectomy; nuclear magnetic resonance imaging; nucleotide sequence; otitis media; polymerase chain reaction; priority journal; restriction fragment length polymorphism; Tsukamurella tyrosinosolven; Actinomycetales; Actinomycetales Infections; Adult; Anti-Bacterial Agents; Bacterial Proteins; Bacterial Typing Techniques; Brain Abscess; DNA Fingerprinting; DNA, Bacterial; DNA, Ribosomal; Female; Heat-Shock Proteins; Humans; Otitis Media; Polymorphism, Restriction Fragment Length; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Tsukamurella tyrosinosolvensjournal article10.1128/JCM.01932-08192975912-s2.0-66149103312