Chang S.-W.Lu P.-Y.Guo J.-H.Tsai T.-C.Tsao Y.-P.SHOW-LI CHEN2019-08-302019-08-3020120042-6822https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855359485&doi=10.1016%2fj.virol.2011.11.015&partnerID=40&md5=c5488b4cf9f0aaf661bd052304244beehttps://scholars.lib.ntu.edu.tw/handle/123456789/416931We previously identified a gene, nuclear receptor-interaction protein (NRIP), which functions as a transcription cofactor in glucocorticoid receptor (GR) and human papillomavirus E2 (HPV E2)-driven gene expression. Here, we comprehensively evaluated the role of NRIP in HPV-16 gene expression. NRIP acts as a transcription cofactor to enhance GR-regulated HPV-16 gene expression in the presence of hormone. NRIP also can form complex with E2 that caused NRIP-induced HPV gene expression via E2-binding sites in a hormone-independent manner. Furthermore, NRIP can associate with GR and E2 to form tri-protein complex to activate HPV gene expression via GRE, not the E2-binding site, in a hormone-dependent manner. These results indicate that NRIP and GR are viral E2-binding proteins and that NRIP regulates HPV gene expression via GRE and/or E2 binding site in the HPV promoter in a hormone-dependent or independent manner, respectively. ? 2011 Elsevier Inc.en-USE2; GR; Hormone; HPV-16; NRIP[SDGs]SDG3dexamethasone; glucocorticoid receptor; protein E2; receptor interacting protein; transcription factor; unclassified drug; virus protein; article; binding site; cancer cell culture; cell proliferation; chromatin immunoprecipitation; complex formation; controlled study; gene expression; gene expression regulation; human; human cell; Human papillomavirus type 16; nucleotide sequence; priority journal; promoter region; protein function; protein protein interaction; receptor binding; reverse transcription polymerase chain reaction; virus genome; Wart virus; Western blotting; Adaptor Proteins, Signal Transducing; Cell Line; DNA-Binding Proteins; Gene Expression Regulation, Viral; Human papillomavirus 16; Humans; Molecular Sequence Data; Nuclear Proteins; Oncogene Proteins, Viral; Papillomavirus Infections; Protein Binding; Receptors, Glucocorticoid; Transcription, Genetic; Up-Regulation; Human papillomavirus; Human papillomavirus type 16journal article10.1016/j.virol.2011.11.015221776992-s2.0-84855359485