2010-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/671450摘要：收集本省之養殖九孔&#30129;疹樣病毒病例，將病毒濃縮及純化分析九孔&#30129;疹樣病毒之基因系列，並完成二次PCR之引子對篩選。 <br> Abstract: Mass mortalities of abalone occurred among cultured abalone in northeastern Taiwan has been reported. Both adult and juvenile abalone suffered from the disease with cumulative mortalities of 70％-80％. However, two different types of clinical signs have been observed in the field including acute and chronic mortality. Histological examination of moribund abalone indicated that the nerve system was the primary target tissue. Transmission electron microscopy examination of cerebral ganglions demonstrated viral particles within the degenerated cells in acute type, however, no viral particles was observed in chronic type. The sequence of herpes-like virus DNA has been study in acute cases. However, primer sets derived from this sequence was unable to amplify significant fragments from chronic cases. In this study, analysis of abalone herpes-like virus using nested PCR will be applied to develop the primer sets to detect the chronic hepes-like virus infection of abalone.九孔&#30129疹樣病毒巢式pcrabaloneherpes-like virusNested PCR