2012-02-132024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/662421摘要：H2O2及乾熱處理對以Xanthomonas campestris pv. Campestri (XCC1-1 strain)污染種子及田間黑腐病株收取之種子皆有良好的減菌效果，藉由處理條件之最佳化調整，可有效殺滅表面攜帶之菌類。乾熱可將處理H2O2後之種子含水量降至與處理前相近。在小規模試驗（數克種子），可在滅菌之同時不影響種子發芽率及幼苗生長；但處理大量種子（1公斤種子）時，滅菌同時卻也降低發芽率。檢討本次試驗，本次種子本身發芽率較差，可能為儲藏過幾年的種子，所以處理後發芽率受影響較大。此外，也可能是在處理大量種子時，處理H2O2時時間因不易控制而延長或烘箱溫度±5oC所造成。因此，本計畫擬對會當年份之種子進行滅菌處理，除乾熱乾燥外，並嘗試以低溫回乾方式乾燥種子， H2O2處理時間也必須更精準，建構種子滅菌之標準流程。<br> Abstract: The protocol of H2O2 treatment followed by heating dry for killing Xanthomonas campestris pv. campestri (XCC) on cabbage seeds were evaluated. H2O2 was able to kill the XCC on either XCC contaminated or field infected seeds. Heating dry was able to lower down the seed water content to the original content. This protocol worked perfectly in small scale experiment (a few grams of seeds) without hurting the germination rate; however, it decreased the germination rate for large scale experiment (1 KG of seeds). The possibly reason for this decrease might be the storage period of these 1 KG field infected seeds. The ±5oC variation of drier and the timing of H2O2 treated duration might be other courses. In the present project, we will focuse on the seeds harvest at current year and try to dry the seed via lower temperature seed drier in addition to the heated dry. A more pr&eacute;cised H2O2 treatment will also be a pursuit. The final goal is to build a standard protocol to disinfect the seeds safely.種傳病害十字花科蔬菜黑腐病十字花科蔬菜黑腐病菌種子溫湯處理種子熱處理seed-borne diseasescrucifer black rotXanthomonas campestris pv. campestrisseed warm water treatmentseed heat treatment