Li, Chia-YangChia-YangLiChao, Louis KuopingLouis KuopingChaoWang, Shu-ChiShu-ChiWangChang, Hon-ZuHon-ZuChangTsai, Min-LungMin-LungTsaiFang, Shih-HuaShih-HuaFangLiao, Pei-ChunPei-ChunLiaoHo, Chen-LungChen-LungHoChen, Shui-TeinShui-TeinChenCheng, Wei-ChungWei-ChungChengChiang, Chi-ShiunChi-ShiunChiangKuo, Yueh-HsiungYueh-HsiungKuoHua, Kuo-FengKuo-FengHuaHsu, Ian C.Ian C.Hsu2012-10-202018-07-062012-10-202018-07-062011http://ntur.lib.ntu.edu.tw//handle/246246/243354Honokiol (HNK) is a phenolic compound isolated from the bark of houpu (Magnolia officinalis), a plant widely used in traditional Chinese and Japanese medicine. While substantial evidence indicates that HNK possesses anti-inflammatory activity, its effect on dendritic cells (DCs) during the inflammatory reaction remains unclear. The present study investigates how HNK affects lipopolysaccharide (LPS)-stimulated human monocyte-derived DCs. Our experimental results show that HNK inhibits the inflammatory response of LPS-induced DCs by (1) suppressing the expression of CD11c, CD40, CD80, CD83, CD86, and MHC-II on LPS-activated DCs, (2) reducing the production of TNF-α, IL-1β, IL-6, and IL-12p70 but increasing the production of IL-10 and TGF-β1 by LPS-activated DCs, (3) inhibiting the LPS-induced DC-elicited allogeneic T-cell proliferation, and (4) shifting the LPS-induced DC-driven Th1 response toward a Th2 response. Further, our results show that HNK inhibits the phosphorylation levels of ERK1/2, p38, JNK1/2, IKKα, and IκBα in LPS-activated DCs. Collectively, the findings show that the anti-inflammatory actions of HNK on LPS-induced DCs are associated with the NF-κB and mitogen-activated protein kinase (MAPK) signaling pathways. ? 2010 Wiley-Liss, Inc.en-US[SDGs]SDG3B7 antigen; CD40 antigen; CD83 antigen; CD86 antigen; gamma interferon; glycoprotein p 15095; honokiol; I kappa B alpha; I kappa B kinase alpha; immunoglobulin enhancer binding protein; interleukin 10; interleukin 12p70; interleukin 13; interleukin 1beta; interleukin 4; interleukin 6; lipopolysaccharide; major histocompatibility antigen class 2; mitogen activated protein kinase; mitogen activated protein kinase 3; synaptophysin; transforming growth factor beta activated kinase 1; tumor necrosis factor alpha; article; cell culture; cell maturation; cell proliferation; cell viability; dendritic cell; enzyme repression; inflammation; monocyte derived dendritic cell; priority journal; protein expression; signal transduction; T lymphocyte; Th1 cell; Th2 cell; Biphenyl Compounds; Cell Differentiation; Cell Line; Cell Proliferation; Cell Survival; Cytokines; Dendritic Cells; Endocytosis; Enzyme Activation; Humans; Inflammation; Lignans; Lipopolysaccharides; MAP Kinase Signaling System; Mitogen-Activated Protein Kinases; Monocytes; NF-kappa B; Phenotype; Th1 Cells; Th2 Cells; Magnolia officinalisjournal article10.1002/jcp.22576http://ntur.lib.ntu.edu.tw/bitstream/246246/243354/-1/57.pdf