國立臺灣大學農藝學系暨研究所劉麗飛2006-07-262018-07-112006-07-262018-07-112003-07-31http://ntur.lib.ntu.edu.tw//handle/246246/19836本計畫是轉殖TPS（海藻糖合成? ） 基因於水稻優良品種台6農7 號 (TNG67)或台梗9 號(TK9)，並在後代中 選育出對乾旱有較強忍受力的水稻。計 畫執行至目前已獲得三種方式調控的 轉殖水稻，這些轉殖水稻大部分為1～ 2 個拷貝，並且可轉錄成mRNA。TPS 蛋白可在體外大量表現，經純化後製成 抗體可進行西方墨點分析。轉殖水稻在 逆境下的表現，初步瞭解可耐鹽，目前 在進行乾旱測試，希望在利用已建立的 耐旱篩選方法中，能獲得耐性較強的植 株。In this project, the TPS gene will be transferred to rice (TNG 67 or TK 9) by Agro-infection methods. We will select the high tolerance of draught in transgenic progeny. Now we obtained transgenic plants that constitutive or stress-inducible promoters drove the TPS gene. The transgenic lines contained a low copy number of transgene that achieved transcription by northern-bolt. The TPS protein was overexpressin in E.coli., therefore will proceed western blot after TPS protein being purified. Our data show the transgenic rice are salt tolerant. The draught tolerant plant will be found as soon as possible.application/pdf799676 bytesapplication/pdfzh-TW國立臺灣大學農藝學系暨研究所海藻糖海藻糖合成酵素基因轉殖水稻TrehaloseTPSgenetransferRicereporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/19836/1/912313B002323.pdf