JUNG-TSU CHENChen, Chein-HungChein-HungChenKu, Ko-LiKo-LiKuHsiao, MichaelMichaelHsiaoCHUN-PIN CHIANGHsu, Tsui-LingTsui-LingHsuMIN-HUEY CHENWong, Chi-HueyChi-HueyWong2021-07-202021-07-202015-10-2000278424https://scholars.lib.ntu.edu.tw/handle/123456789/572082The incidence and mortality rate of oral cancer continue to rise, partly due to the lack of effective early diagnosis and increasing environmental exposure to cancer-causing agents. To identify new markers for oral cancer, we used a sialylation probe to investigate the glycoproteins differentially expressed on oral cancer cells. Of the glycoproteins identified, B7 Homolog 3 (B7-H3) was significantly overexpressed in oral squamous cell carcinoma (OSCC), and its overexpression correlated with larger tumor size, advanced clinical stage, and low survival rate in OSCC patients. In addition, knockdown of B7-H3 suppressed tumor cell proliferation, and restoration of B7-H3 expression enhanced tumor growth. It was also found that the N-glycans of B7-H3 from Ca9-22 oral cancer cells contain the terminal α-galactose and are more diverse with higher fucosylation and better interaction with DC-SIGN [DC-specific intercellular adhesion molecule-3 (ICAM-3)-grabbing nonintegrin] and Langerin on immune cells than that from normal cells, suggesting that the glycans on B7-H3 may also play an important role in the disease.enCD276; fucose; glycan sequencing; proliferation; terminal α-galactoseCD276; Fucose; Glycan sequencing; Proliferation; Terminal α-galactose[SDGs]SDG3galactose; glycan; glycoprotein; intercellular adhesion molecule 3; langerin; tumor marker; B7 antigen; CD276 protein, human; animal cell; animal experiment; animal model; animal tissue; cancer cell; cancer patient; cancer staging; cell proliferation; Conference Paper; controlled study; fucosylation; gene silencing; glycosylation; immune response; mouse; mouth cancer; mouth squamous cell carcinoma; nonhuman; priority journal; protein expression; sialylation; survival rate; tumor cell; tumor growth; tumor volume; glycosylation; human; immunology; metabolism; mouth tumor; pathology; squamous cell carcinoma; B7 Antigens; Carcinoma, Squamous Cell; Cell Proliferation; Glycosylation; Humans; Mouth Neoplasmsjournal article10.1073/pnas.1516991112264388682-s2.0-84947484053https://scholars.lib.ntu.edu.tw/handle/123456789/568972