倪衍玄2006-07-262018-07-112006-07-262018-07-112003http://ntur.lib.ntu.edu.tw//handle/246246/22880A handy in vitro viral replication system is mandatory for hepatitis B virus (HBV) study. A recombinant baculovirus with 1.3XHBV DNA construct was previously designed to infect HepG2 cells. We adapted this system and set up another one using 1.5XHBV DNA construct to generate our recombinant baculovirus, and we use Huh7 cells instead of HepG2 cells to establish this system. HBV genome was inserted into the baculovirus by recombination and the novel HBV recombinant baculovirus was identified by enzyme digestion. The viral stock was purified and its titre was determined. We then use the HBV recombinant baculovirus to infect Huh7 cell culture and demonstrate its ability to produce HBsAg. The produciton of HBsAg was first detected in the media three days after infection. Its production was in proportion to the loading amount of HBV recombinant baculovirus. A sustained HBsAg production could be achieved by superinfection of this recombinant virus to the already infected Huh7 cell culture. This system can be applied to the basic and clinical studies of HBV.application/pdf153786 bytesapplication/pdfzh-TW國立臺灣大學醫學院小兒科hepatitis B virusbaculovirus[SDGs]SDG3reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/22880/1/912314B002152.pdf