CHIN-CHUNG SHUWu M.-F.JANN-YUAN WANGHSIN-CHIH LAILI-NA LEEBOR-LUEN CHIANGCHONG-JEN YU2020-07-032020-07-0320171465-9921https://scholars.lib.ntu.edu.tw/handle/123456789/508186Background: Tuberculosis (TB) is one of the most common infectious diseases worldwide. During active tuberculosis, T helper (Th) 17 cells are decreased, however the association with inhibitory immune regulation is unclear. Methods: We enrolled 27 patients with TB and 20 age- and sex-matched controls and studies their lymphocyte status. Peripheral blood lymphocytes were isolated and programmed death-1 (PD-1) and programmed death ligand 1 (PD-L1) were measured on Th17 cells by using flow cytometry after the cells were stimulated with phorbol 12-myristate 13-acetate and ionomycin for 6 h. In addition, Th2 and regulatory T cells were measured and analyzed. Results: The TB group had lower levels of Th17 cells but higher levels of Th2 and Treg cells than the controls. In Th17 cells, the percentage of PD-L1 was higher in the TB group than that in the controls. In Th2 and Treg cells, the percentage of cytotoxic T-lymphocyte associated protein 4 (CTLA-4) was lower in the TB group and PD-1 was higher in Treg cells in the TB group. In the patients with extra-pulmonary TB, levels of Th1, Th2 and T17 cells were lower than those with pulmonary TB. The percentage of PD-1 on Th1 lymphocytes positively correlated with radiographic score. Conclusions: Lower level of Th17 in TB patients may be associated with increased percentage of PD-L1 and increasing levels of Th2 and Treg cells which influenced by CTLA-4. ? 2017 The Author(s).[SDGs]SDG3cytotoxic T lymphocyte antigen 4; programmed death 1 ligand 1; programmed death 1 receptor; tuberculostatic agent; CD274 protein, human; programmed death 1 ligand 1; adult; age; Article; clinical article; controlled study; extrapulmonary tuberculosis; female; health status; human; human cell; lung tuberculosis; lymphocyte count; male; Mycobacterium tuberculosis; nonhuman; peripheral lymphocyte; radiodiagnosis; radiographic score; regulatory T lymphocyte; scoring system; sex; T lymphocyte activation; Th17 cell; Th2 cell; aged; blood; immunology; isolation and purification; metabolism; middle aged; prospective study; regulatory T lymphocyte; Th17 cell; Th2 cell; tuberculosis; Adult; Aged; B7-H1 Antigen; Female; Humans; Male; Middle Aged; Mycobacterium tuberculosis; Prospective Studies; T-Lymphocytes, Regulatory; Th17 Cells; Th2 Cells; Tuberculosisjournal article10.1186/s12931-017-0580-3286515762-s2.0-85021184873