2013-03-052024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/663047摘要：番茄黃化捲葉病對番茄造成的經濟損失可高達 100%，對全球番茄的產業是一大威脅。番茄黃化捲葉病的致病病原為眾多親緣相近的番茄黃化捲葉病毒。在病毒分類上，番茄黃化捲葉病毒屬於豆類金黃嵌紋病毒屬 (Begomovirus)，雙生病毒科 (Geminiviridae)。此類群病毒僅依賴菸草粉蝨傳播，菸草粉蝨以持續性增殖型傳播模式傳播 begomoviruses，所以病毒會感染粉蝨腸道細胞並於粉蝨體內循環。根據田間調查結果顯示番茄捲葉台灣病毒 (Tomato leaf curl Taiwan virus, ToLCTWV) 於 2007 年有接近 100% 的高流行率，很快地在 2009 年就被番茄黃化捲葉泰國病毒 (Tomato yellow leaf curl Thailand virus, TYLCTHV) 所取代。通常病毒的結構蛋白在病毒進入昆蟲的中腸細胞的過程中，扮演著重要的角色。前人研究顯示，begomoviruses 的鞘蛋白 (coat protein) 與病毒於粉蝨體內循環具有緊密的關聯性。我們假設經由體外合成 (in vitro synthesis) 之 TYLCTHV 鞘蛋白，被病媒昆蟲取食後，能阻斷 begomoviruses 和病媒昆蟲體內受體 (receptor) 的結合或與 begomoviruses 競爭受體的結合位，便可以用來抑制病毒的傳播。本計畫研究目的有三：1. 檢測受 TYLCTHV 感染的菸草粉蝨組織；2. 鑑別與 TYLCTHV 的病媒傳播有關的病毒蛋白；3. 利用重組病毒蛋白以發展病媒專一性之植物病毒病害防治策略。<br> Abstract: Tomato yellow leaf curl disease (TYLCD) can cause yield losses as high as 100% in tomato and is emerging as a serious threat to global tomato production. TYLCD is associated with a group of phylogenetically related viruses referred to as tomato yellow leaf curl viruses. These viruses belong to the genus Begomovirus of the family Geminiviridae and are obligatorily transmitted by Bemisia tabaci. Bemisia tabaci transmits begomoviruses in a persistent-propagative mode, so begomoviruses can infect midgut cells and circulate in whitefly body. A survey revealed the progressive displacement of Tomato leaf curl Taiwan virus (ToLCTWV) by Tomato yellow leaf curl Thailand virus (TYLCTHV) from 2007 to 2009 in tomato fields in Taiwan. Virus structure protein plays an important role for plant viruses entering insect midgut cells, the first site of infection. Previous studies showed that the coat protein of begomoviruses is correlated to the virus circulation in B. tabaci. We hypothesize that in vitro synthesis of the coat protein of TYLCTHV can interrupt the binding between virus and receptor on insect cells or compete the virus binding sides of the receptor. Therefore, virus transmission by whiteflies is suppressed. The objectives of this grant proposal are to: 1. detect TYLCTHV-infected organs in B. tabaci; 2. identify the protein of TYLCTHV related to vector transmission; 3. develop a vector-specific control strategy to plant viral diseases using recombinant viral proteins.菸草粉蝨豆類金黃嵌紋病毒屬鞘蛋白病媒防治Bemisia tabaciBegomovirusCoat proteinVector control