2008-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/694431摘要：牛乳含有豐富的蛋白質、礦物質及維生素。不僅具有極高的營養價值，亦有多種的生理功用。因此，乳蛋白質及其衍生肽被廣泛應用於食品及醫藥工業。乳中許多蛋白質具有「生物活性」，其中以免疫球蛋白最具代表性。乳中除了生物活性蛋白質以外，亦有諸多生物活性肽。其主要由不具生物活性之乳蛋白質經酵素水解、消化或加工過程而產生。乳生物活性肽種類繁多且作用廣泛，其生理功能包括抗菌、抗高血壓、抗血栓、免疫調節，以及促進鈣質吸收。因此，自乳中開發生物活性肽之新型產品將使乳蛋白質之應用更為多元化，不僅可提昇畜牧業之競爭力，對食品及醫藥工業亦有所助益。本研究擬自乳蛋白質中尋找生物活性肽，並利用基因重組技術以微生物大量產製生物活性肽，生產具經濟效益的飼料添加物，期能取代抗生素的使用。全程計畫內容將包含生物活性肽篩選、以益生菌表達生物活性肽、人造油體法純化生物活性肽等工作項目。本年度將把生物活性肽基因接合於油體膜蛋白基因，並選殖於大腸桿菌表達質體中，進行基因之大量表現。再利用人造油體法進行生物活性肽之純化，以評估生物活性肽之產量及抗菌性。<br> Abstract: Milk provides a rich source of valuable proteins, minerals and vitamins. The nutritional significance of the protein fraction has a macronutritient as well as a physiological aspect. These aspects are important for the use of milk proteins and milk-protein-derived peptides in dietary and pharmaceutical applications. Milk contains various components with physiological functionality. The term “bioactivity” is used in connection with such components. Immunoglobulins are a well-known example of bioactive milk proteins. Besides bioactive proteins, milk also provides bioactive peptides. Milk-protein derived bioactive peptides are inactive within the sequence of the parent protein and can be released and activated by enzymatic proteolysis, gastrointestinal digestion, or food processing. These peptides can exert a wide range of effects, such as antimicrobial, antihypertensive, antithrombotic, and immunomodulatory, in addition to aiding in the mineral absorption of calcium. Therefore, exploiting new products from milk bioactive peptides not only increases the utilization of milk proteins but also promote the development of livestock, food, and health care industries. This study is conducted to screen out the bioactive peptides from milk proteins and use DNA recombinant technology to clone these bioactive peptide genes into expression vectors. The milk-derived bioactive peptides produced by the recombinant host could be used as a feed or food additive. This study includes three tasks: milk-protein derived bioactive peptides screening, expression of the bioactive peptide genes by probiotics, and purification of the recombinant bioactive peptides via artificial oil bodies (AOBs). This year, we propose to produce bioactive peptides via AOBs. The bioactive peptide genes will be constructed to fuse to oleosin gene. The fusion genes will be overexpressed in E. coli and used to reconstitute AOBs. Finally, the recombinant bioactive peptides will be subsequently released from AOBs and estimated the anti-microbial activities.生物活性肽人造油體抗菌活性bioactive peptideartificial oil bodyantimicrobial activity以基因工程技術產製生物活性肽(III)利用人造油體大量產製生物活性肽