2009-04-022024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/661880摘要：樟芝(Antrodia cinnamomea)是臺灣特有之珍貴藥用菌種，目前已有一些研究報告指出，樟芝具有抗癌或調節免疫等多方面之活性。此外，由於它僅生長於保育類牛樟的中空心材內壁上，因此，在野生樟芝子實體數量日益不足的情況下，如何有效的人工培養，即為目前研究開發之重要工作。本研究計畫將利用已建構完成之基因資料庫，鑑定不同品系及分析不同發酵階段基因表現的情形，選殖有效成分(可能為多醣類與三&#33820;類等)生合成等之相關基因，期望未來在代謝體學、蛋白質體學、基因晶片及生物資訊學等配合下，尋找與樟芝生長分化和有效二次代謝物生合成有關之基因表現，進而建立分子標記、品管產品之有效性，開發具科學基礎之保健產品。<br> Abstract: Chang-Zhi (Antrodia cinnamomea) is an endemic fungal species grown in Taiwan. A. cinnamomea has been reported to have many bioactivities, including anticancer, immunomodulating activities, and so on. However, the species is restricted to grow from Cinnamomum kanehirai Hay (Lauraceae) and cannot be large-scale cultivated artificially until now. Consequently, it is becoming important to yield effective products by artificial cultivation. In our current investigation, we have established the EST libraries. Thus, the strain identification and differential expression will be analyzed to clone the related genes to polysaccharides and triterpene synthesis. Coupling with metabolomics, proteomics, transgenic technology and bioinformatics, we hope in the future to explore the genes, which involved in the biosynthesis of biologically effective metabolites. And the molecular marker system of A. cinnamomea will be established to control the quality of the products. We believe that the results obtained in this study would provide the useful technology and develop solid science-based health products in the future.樟芝生合成分子標記二次代謝Antrodia cinnamomeabiosynthesisgenetic markersecondary metabolites