Roth, RonelleRonelleRothChiapello, MarcoMarcoChiapelloMontero, HéctorHéctorMonteroGehrig, PeterPeterGehrigGrossmann, JonasJonasGrossmannO’Holleran, KevinKevinO’HolleranHartken, DeniseDeniseHartkenWalters, FergusFergusWaltersSHU-YI YANGHillmer, StefanStefanHillmerSchumacher, KarinKarinSchumacherBowden, SarahSarahBowdenCraze, MelanieMelanieCrazeWallington, Emma J.Emma J.WallingtonMiyao, AkioAkioMiyaoSawers, RuairidhRuairidhSawersMartinoia, EnricoEnricoMartinoiaPaszkowski, UtaUtaPaszkowski2019-07-222019-07-222018-12-012041-1723https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056288432&doi=10.1038%2fs41467-018-06865-z&partnerID=40&md5=827f956afbeabe028220e16dc0267789https://scholars.lib.ntu.edu.tw/handle/123456789/414328© 2018, The Author(s). In terrestrial ecosystems most plant species live in mutualistic symbioses with nutrient-delivering arbuscular mycorrhizal (AM) fungi. Establishment of AM symbioses includes transient, intracellular formation of fungal feeding structures, the arbuscules. A plant-derived peri-arbuscular membrane (PAM) surrounds the arbuscules, mediating reciprocal nutrient exchange. Signaling at the PAM must be well coordinated to achieve this dynamic cellular intimacy. Here, we identify the PAM-specific Arbuscular Receptor-like Kinase 1 (ARK1) from maize and rice to condition sustained AM symbiosis. Mutation of rice ARK1 causes a significant reduction in vesicles, the fungal storage structures, and a concomitant reduction in overall root colonization by the AM fungus Rhizophagus irregularis. Arbuscules, although less frequent in the ark1 mutant, are morphologically normal. Co-cultivation with wild-type plants restores vesicle and spore formation, suggesting ARK1 function is required for the completion of the fungal life-cycle, thereby defining a functional stage, post arbuscule development.en[SDGs]SDG15journal articlehttps://api.elsevier.com/content/abstract/scopus_id/8505628843210.1038/s41467-018-06865-z304100182-s2.0-85056288432WOS:000449494100002https://api.elsevier.com/content/abstract/scopus_id/85056288432