MING-KWANG SHYUChen C.-W.NENG-YU LINLiao W.-C.CHI-HAU CHENCHUN-JUNG LINHuang H.-C.Lee Jr. J.Huang M.-J.Tseng G.-F.JIN-CHUNG SHIHCHIEN-NAN LEEFON-JOU HSIEHMIN-CHUAN HUANG2020-03-022020-03-022011-120021-972Xhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-83155187296&doi=10.1210%2fjc.2011-1368&partnerID=40&md5=ae15d05d5dad0fa1fec58456031afaf3https://scholars.lib.ntu.edu.tw/handle/123456789/467191Context: Preeclampsia is a pregnancy-specific disorder that features insufficient extravillous trophoblast (EVT) invasion. We have previously shown that MUC1 expression in human placenta increases with gestational age and inhibits choriocarcinoma cell invasion. Objective: Here, we studied whether MUC1 expression in preeclamptic placentas is dysregulated and the mechanism of EVT invasion regulated by MUC1. Design: MUC1 expression in severe preeclamptic placentas and gestational age-matched control placentas was analyzed by real-time RT-PCR, Western blot analysis, and immunohistochemistry. The effects of MUC1 expression on cell-matrix adhesion, invasion, and cell signaling were studied in HTR8/SVneo EVT cells. Results: We found that MUC1 mRNA and MUC1 protein were significantly up-regulated in severe preeclamptic placentas when compared with the gestational age-matched control placentas. Immunohistochemical analyses showed increased expression of MUC1 in the syncytiotrophoblast and EVT of severe preeclamptic placentas. In addition, MUC1 overexpression suppressed cell-matrix adhesion and invasion of EVT cells. Importantly, our data showed that MUC1 overexpression inhibited β1-integrin activity and phosphorylation of focal adhesion kinase, whereas the surface expression of β1-integrin was not significantly changed. Conclusions: Our findings suggest that MUC1 is overexpressed in severe preeclamptic placentas and that MUC1 overexpression suppresses EVT invasion mainly via modulating β1-integrin signaling. Copyright ? 2011 by The Endocrine Society.[SDGs]SDG3beta1 integrin; focal adhesion kinase; messenger RNA; mucin 1; article; cell adhesion; cell communication; cell invasion; disease severity; enzyme phosphorylation; extracellular matrix; extravillous trophoblast; female; gestational age; human; human cell; human tissue; immunohistochemistry; placenta; preeclampsia; priority journal; protein expression; real time polymerase chain reaction; syncytiotrophoblast; trophoblast; upregulation; Western blotting; Adult; Antigens, CD29; Cell Adhesion; Cell Movement; Cells, Cultured; Female; Humans; Mucin-1; Placenta; Pre-Eclampsia; Pregnancy; Signal Transduction; Trophoblasts; Up-Regulationjournal article10.1210/jc.2011-1368219178662-s2.0-83155187296