The 27-bp tandem repeat polymorphism in intron 4 of the endothelial nitric oxide synthase gene is not associated with coronary artery disease in a hospital-based Taiwanese population

HWANG, JUEY-JENJUEY-JENHWANGCHIA-TI TSAIHUEI-MING YEHFU-TIEN CHIANGHsu K.-L.Tseng C.-D.Liau C.-S.Tseng Y.-Z.LING-PING LAI2020-12-302020-12-3020020008-6312https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036238452&doi=10.1159%2f000057674&partnerID=40&md5=90ac873ff22e5ebcfce7bd6cb5b51c2ehttps://scholars.lib.ntu.edu.tw/handle/123456789/536582We studied whether the 27 base pair (bp) tandem repeat polymorphism in intron 4 of the endothelial nitric oxide synthase (eNOS) gene was associated with coronary artery disease (CAD) in a hospital-based Taiwanese population. We included 219 consecutive patients who underwent coronary angiography at our institution. Two alleles, containing 4 (eNOS4a) and 5 repeats (eNOS4b), were identified after polymerase chain reaction amplifying intron 4 of the eNOS gene. The genotype frequencies for eNOS4b/b, eNOS4a/b and eNOS4a/a were 77.9, 21.5 and 0.6% in CAD subjects, and 80, 20 and 0% in control subjects (Fisher's exact test, p = 0.90), respectively. The odds ratio (OR) for CAD in patients with at least one eNOSa allele was 1.2 (0.5-2.9) after adjustment for classical CAD risk factors. The eNOS4a allele was not associated with the severity of CAD (Fisher's exact test, p = 0.90) and the occurrence of acute myocardial infarction (AMI) or unstable angina (adjusted OR 0.6, 0.3-1.6) in patients with CAD. In conclusion, the 27-bp repeat polymorphism of the eNOS gene was not associated with CAD and the occurrence of AMI or unstable angina in a hospital-based Taiwanese population. Copyright ? 2002 S. Karger AG, Basel.[SDGs]SDG3nitric oxide synthase; acute heart infarction; adult; aged; angiocardiography; article; base pairing; controlled study; coronary artery disease; coronary risk; disease severity; female; gene frequency; genetic polymorphism; genotype; hospital; human; intron; major clinical study; male; polymerase chain reaction; priority journal; risk factor; Taiwan; tandem repeat; unstable angina pectoris; Aged; Case-Control Studies; Coronary Arteriosclerosis; Female; Genotype; Humans; Introns; Male; Middle Aged; Myocardial Infarction; Nitric Oxide Synthase; Nitric Oxide Synthase Type III; Odds Ratio; Polymerase Chain Reaction; Polymorphism, Genetic; TaiwanThe 27-bp tandem repeat polymorphism in intron 4 of the endothelial nitric oxide synthase gene is not associated with coronary artery disease in a hospital-based Taiwanese populationjournal article10.1159/000057674119789512-s2.0-0036238452