Tseng, C.-H.C.-H.TsengHSIANG-JUNG TSAIChang, C.-M.C.-M.Chang2018-09-102018-09-102014http://www.scopus.com/inward/record.url?eid=2-s2.0-84904117526&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/387406Introduction. The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing. Method. This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes. Result and Conclusion. This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research. ? 2014 Chun-Hsien Tseng et al.[SDGs]SDG3hemagglutinin; sialidase; hemagglutinin, avian influenza A virus; Influenza virus hemagglutinin; organic compound; sialidase; SYBR Green I; article; avian influenza; avian influenza virus; diagnostic procedure; health survey; human; molecular diagnosis; nonhuman; nucleotide sequence; real time polymerase chain reaction; sequence analysis; virus strain; animal; avian influenza; bird; chemistry; classification; DNA sequence; genetics; molecular pathology; polymerase chain reaction; procedures; reverse transcription polymerase chain reaction; virology; virus genome; Animals; Birds; Genome, Viral; Hemagglutinin Glycoproteins, Influenza Virus; Influenza in Birds; Neuraminidase; Organic Chemicals; Pathology, Molecular; Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNAjournal article10.1155/2014/653056