潘永寧Pan, Yung-Ning臺灣大學:機械工程學研究所吳恩育Wu, En-YuEn-YuWu2010-06-302018-06-282010-06-302018-06-282009U0001-1708200915270200http://ntur.lib.ntu.edu.tw//handle/246246/187232本研究針對Ti-Nb基合金以氬氣(Ar)及氧氣(O2)電漿處理來進行表面處理，研究方法上，首先以Ar電漿清除鈦合金表面可能存在之微量化學污染物及雜質，然後以O2電漿處理，並探討電漿能量對於表層之物理化學性質的影響。經電漿處理後之Ti-Nb基底合金表面形貌及氧化層特性分別以掃瞄式電子顯微鏡(SEM)、穿透式電子顯微鏡(TEM)、X光粉末繞射儀(XRPD)及X光光電子能譜儀(XPS)進行分析。另外，氧化層厚度和合金表面之潤濕性則以二次離子質譜儀(SIMS)及液滴法(sessile drop method) 進行量測。此外，以骨母細胞培養、白蛋白吸附及血液凝固性的實驗中，評估生物相容性。究結果顯示，Ti-Nb基合金經由氧氣電漿表面處理後，會形成一較厚之氧化層，此氧化層的組成主要以TiO2和Nb2O5為主，而氧化層的厚度會隨著電漿能量的增加而增加，並提升合金表面之潤濕性，均有利於骨母細胞的繁殖演化。於血液凝固的研究結果得知，經由氧氣電漿表面處理後，血液快速在鈦合金表面凝固，形成粗長狀的纖維蛋白凝塊，並將紅血球網羅在內。此外，白蛋白(albumin)吸附結果亦顯示，經丙烯胺(Allylamine)電漿處理及交鏈劑戊二醛(Glutaraldehyde)的連接(crosslink)處理後，有助於白蛋白在鈦鈮合金表面的附著。綜上所述，O2及丙烯胺電漿處理可視為一個有效的Ti-Nb合金表面改質的方式，可得到較佳的細胞能力和癒合速度，進一步加速植體與骨組織之間的骨整合。This study employed low temperature plasma treatment to modify the surfaces of Ti-Nb based alloy. Firstly, argon plasma treatment is used to clean the contamination and impurity on the alloy surface, and then oxygen plasma treatment was performed to induce the formation of the oxide layer on the alloy surface. Scanning electron microscopy (SEM), grazing incident X-ray diffraction (GIXRD) and electron spectroscopy for chemical analysis (XPS) were employed to analyze the characteristics of the oxide layer formed by plasma treatment. The thickness and wettability of oxide layers on the surface of the titanium alloy were measured by secondary ion mass spectrometer (SIMS) and contact angle, respectively. In vitro study, the compatibility was evaluated by the parameter of cell culture, albumin adsorption and blood culture.he results show that the oxide layer was formed by oxygen plasma treatment and the main compositions are TiO2 and Nb2O5. Both the thickness and surface wettability of oxide layers increase with increasing plasma power. The results show that the oxidation effect on the alloy surface brought about by O2-plasma treatment enhances the proliferation and spreading of cells. The treated surfaces had been found to improve the clotting time and to introduce the formation of a more extensive and three dimensionally complex fibrin clots. In addition, the formation of nitrides by allylamine plasma treatment provides amino-group linkings for the attachment of albumin. In addition, after O2-plasma treatment, some specimens were further processed by allylamine plasma treatment and crosslinking with glutaraldehyde, which exhibited increased protein adsorption. Ti-Nb alloy with O2-plasma and allylamine plasma treatment is believed to attain better ability of cells and tissue healing and therefore promote osseointergration.誌謝...............................ii要...............................iiibstract ..........................v錄...............................vii目錄.............................x目錄.............................xi一章 緒論.1 前言...........................1.2 研究動機.......................1二章 文獻回顧.1 生醫材料.......................4.2 生醫材料性質之要求.............4.3 生醫用金屬之骨植入材的缺點.....4.4 鈦植入材之表面處理.............7.5 電漿表面處理………………………8.5.1 電漿的形成...................8.5.2 電漿種類.....................9.5.3 電漿表面處理特點.............10.6 電漿表面改質技術...............11.6.1 輝光放電.....................11.6.2 電漿離子佈植.................12.6.3 電漿噴鍍.....................12.6.4 電漿沉積.....................13.6.5 電漿聚合.....................13.6.6 電漿接枝聚合.................13.6.7 電漿蝕刻.....................14.7 氧氣電漿.......................14.8 丙烯胺電漿.....................15三章 實驗步驟及方法.1合金準備........................22.2試片準備及表面處理..............22.3試片分析........................23.3.1基材組成元素分析..............23.3.2試片表面形貌..................23.3.3試片表面成分分析..............23 3.3.4試片表面性質分析…………24.4 骨細胞性質分析.................25 3.4.1 細胞形貌................25 3.4.2 細胞數目................26 3.4.3 細胞活性................27.5 血液凝固性分析………………………27 3.5.1 凝血時間分析…………………28 3.5.2 紅血球分析……………………28.6 白蛋白吸附分析..................28四章 Ti-30Nb-1Fe-1Hf之O2電漿效應.1 表面形貌觀察....................34.2 氧化層形貌觀察..................35.3 潤濕性分析......................36.4 化合物構造分析..................37.4.1 XRPD分析......................37.4.2 XPS分析.......................38.5 細胞培殖分析....................40五章 Ti-40Nb-1Hf之O2電漿效應.1 表面形貌觀察....................57.2 氧化層形貌觀察..................57.3 潤濕性分析......................57.4 化合物構造分析..................58.4.1 XRPD分析......................58.4.2 TEM分析………………………………58.4.3 XPS分析.......................59.5 細胞培殖分析....................60.6血液培殖分析……………………………61 5.6.1凝血速度分析……………………61 5.6.2血液凝結物分析…………………63.7白蛋白吸附分析…………………………64六章 討論..........................82七章 結論..........................89考文獻.............................91作.................................975634953 bytesapplication/pdfen-US鈦鈮合金電漿處理骨整合Ti-Nb alloyPlasma treatmentOsseointergrationthesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/187232/1/ntu-98-D92522004-1.pdf