Chen Y.-HYu J.-FChang Y.-JChin S.-CWang L.-CLin H.-LPEI-SHIUE TSAILIH-CHIANN WANG2021-07-262021-07-26202020762615https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092345675&doi=10.3390%2fani10101825&partnerID=40&md5=c330d9fb632b08058114cef4c3e56ba6https://scholars.lib.ntu.edu.tw/handle/123456789/573178Semen collection can be achieved via hand penile massage or rectal stimulation using electro-ejaculation methods. Traditional electro-ejaculation procedure applied relatively high voltage of 3–15 volts with a maximum current of 900 mA. However, these manipulations often result in great stress and discomforts in animals. In this study, we showed low-voltage electro-ejaculation procedure using 2–3 volts with a maximum current of 500 mA can efficiently stimulated ejaculations in zoo captive lanyu miniature pigs with a high success rate of 81.3% (13/16). Besides normal semen properties (semen volume, pH, sperm concentration), we demonstrated that low-voltage electro-ejaculation caused less stress in the animals, and sperm cells obtained via low-voltage electro-ejaculation exhibit low abnormality (10.3%), high viability (84.3%), motility (75.7%), progressive motility (63.7%), and acrosome integrity (88%). However, cryopreservation protocol used in the current study requires further optimization, as sperm mitochondrial function was partially compromised during freezing procedures. Taken together, we demonstrated in this study that a low-voltage electro-ejaculation approach can be used to obtain quality sperm cells from zoo captive lanyu miniature pig with less physical stress during electro-ejaculation procedure. ? 2020 by the authors. Licensee MDPI, Basel, Switzerland.acrosome; article; cell viability; cryopreservation; ejaculation; human cell; male; minipig; mitochondrion; nonhuman; physical stress; pig; seminal plasma; spermatozoon density[SDGs]SDG3journal article10.3390/ani101018252-s2.0-85092345675