2012-11-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/659472摘要：醫學科技日新月異，加上表面改質技術的開發，生醫植入物的功能與效用從以機械性質為主的 需求，漸漸提升至同時具有生物積極性的方面發展。以鈦合金鋼板為本計劃研究重點，骨科鋼板手 術最大併發症是發生感染造成骨髓炎。骨髓炎之產生有二途徑；其一是細菌經由血液帶至骨折處， 其二是細菌附著於鋼板上就地繁殖。途徑一可以藉由抗生素加以殺菌，途徑二依據人體的滅菌殺菌 機制，包括白血球吞噬細菌及抗生素使用，都無法完全有效的殺死這些附著在鋼板上的細菌。本計 劃嘗試在鈦合金鋼板上覆蓋一層抗菌物質，期待藉此方式能阻斷細菌經由附著在鋼板上侵入人體的 途徑，降低骨髓炎產生的機會。奈米銀粒子具有公認的抗菌效果，可殺死600多種細菌，且為重要的奈米材料之一，其應用面廣 泛包含了生物、醫藥，生醫材料、化學、化工、奈米複合材料等。另外，奈米銀粒子已知具有抑菌性 質，可與病原菌之細胞壁/膜結合後進入菌體使其失活，此獨特作用機制已被廣泛地應用於臨床醫療 上；尤其奈米銀粒子對於抗藥性菌株也具有抗菌能力的特性，目前更受到高度重視。但是在製備的過 程中，因為奈米尺寸的效應以及銀粒子本身的凡得瓦爾力(van der Waalsforce)的親合性，導致其均勻 分散之困難性（尤其是分散於溶劑或製成粉體），奈米銀顆粒更容易因聚集造成其生物之毒性，以致 在製程上無法有效提高濃度。因此本計晝預計以一年的時間完成下列相關之研究工作。前半年的研究重點在奈米銀粒子大小之 操縱、及對熱、氧化、UV、製程之穩定性問题，因此控制奈米銀粒子的大小、穩定性、分散性為研 究之重要目標。而後半年則針對奈米銀/奈米矽片混摻聚乙烯醇進行鋼板的表面改質，藉由聚乙烯醇 的黏著劑性質來混合具有抗菌效果的奈米銀粒子。該研究重點著重於複合材料的基本物理化學性質分 析、細胞毒性測試以及抗菌功效分析，藉此計晝來確認該複合材料可真正運用於人體植入物之鋼板表 面，增加鋼板的抗菌，更加以提升鋼板的經濟價值。在此計晝中將了解奈米複合材料對於抗菌效果的成效，具材料前瞻性與共通性，預期研發成果將 涵蓋奈米材料科技與生醫科技等，產出豐碩成果與相關專利。<br> Abstract: Titanium plates were used widely in the treatment of human bone fracture for recent ten years. The most serious complication in this plating procedure is infection and finally leaded to osteomyelitis. There are two major routes for invasion of bacteria. The first route is through blood stream. The bacteria invaded the human body everywhere even though a small wound. Following the blood stream, they seeded to the fracture site. The second route is through the metal implant itself. The bacteria will attach on the surface of metal implant and proliferate in situ. If the bacteria invaded the human body by blood steam, we can use antibiotics to kill them. But if the bacteria invaded by metal implant, the leucocytes cannot kill these bacteria due to the metal surface is the place that the circulation system cannot reach. And the antibiotics also lose effect due to the same reason. In this proposal, we try to develop the new material and technique to improve this situation.Silver nanoparticles (AgNP) have emerged as an important field that have a wide range of applications such as biological, medicine, biomaterials, chemistry, chemical engineering and nanocomposites uses. The antimicrobial activity of AgNP largely has been studied with human pathogenic bacteria, especially in silver-ion resistance strain. The mechanism has been suggested because the AgNP destabilizes the outer membrane, collapses the plasma membrane potential and decreases ATP. However, the use of AgNP in the past has been limited by their instabilities of aggregations into larger particles (especially dispersing in solvent system or in powder form), oxidation into silver ions and other adverse effects.This project intended to be a year to complete related research work. The first half of the research focus will be preparation of AgNP/NSP nanohybrids, especially focus on the property of heat, oxidation stress, UV resistance and the stability of the process. And the study after six months will investigate the roles of AgNP/NSP-PVA nanohybrids on surface modification in steel plate. In bio-application, this project will investigate the roles of AgNP/NSP-PVA nanohybrids on the biological behaviors; especially focus on the chemical-physical analysis, cytotoxicity and antimicrobial ability. The relationship between the surface morphology and biocompatibility will future explore in human implantation.This plan combined with scientific and technology of “polymer materials”，“Biochemistry” and “tissue engineering” blueprint.奈米矽片銀聚乙烯醇細胞毒性抗菌性骨折骨髓炎鈦合金鋼板AgNP/NSPpolyvinyl alcoholcytotoxicityantibacterial activitybone fractureosteomyelitistitanium plate