Shu-Jen ChiangPo-Hang TsengMei-Chun LinChih-Yu LeuMadhusudan RoyChen-Yuan DongPei-Jen LouBrian J. F. WongJustus F. Ilgner2024-10-082024-10-082024-03-13https://scholars.lib.ntu.edu.tw/handle/123456789/721826At present, the administration of chemotherapy drugs is largely based on physician experience and clinical guidelines. Clearly, a personalized solution would significantly benefit patients in the improvement of clinical outcomes. In this work, we used a spectroscopic approach in which a water-soluble tetrazolium salt (WST-8) assay was applied to patient-derived slice organoids. WST-8 would react with intracellular dehydrogenase resulting in the formation of formazan dye. The absorption at 450nm can be used to assess the viability of the organoids 5 days after initiation of organoid culture. With additional development, this approach may be used to improve the clinical outcome of patients undergoing chemotherapy.[SDGs]SDG3[SDGs]SDG6conference paper10.1117/12.2691213